Oecludin在胃食管反流病患者食管上皮细胞中的表达

目的研究胃食管反流病患者食管上皮细胞Oecludin表达的变化。方法选取62例胃食管反流病患者，其中反流性食管炎（RE）30例（RE组）、非糜烂性胃食管反流病（NERD）32例（NERD组），采用免疫组化技术对其食管下段上皮的Oecludin分布表达进行检测，并以10例正常食管上皮作为对照组。结果对照组Oecludin均正常表达。NERD组4例正常表达，28例不同程度异常表达，为细胞膜不完整表达、细胞质表达及表达缺失。RE组均异常表达；RE组和NERD组与对照组0ccludin异常表达阳性率比较差异有统计学意义（p〈0．01），而RE组与NERD组异常表达阳性率比较差异无统计学意义（P〉0．05）。结论胃食管反流病患者食管上皮Oecludin数量减少、分布异常，影响黏膜上皮屏障的完整性，可能是胃食管反流病的发病机制之一，而RE与NERD患者Oecludin异常表达无明显差异。

The study on Oecludin in esophageal epithelium in gastroesophageal reflux disease

Objective To investigate the distribution and expression of Occludin in esophageal epithelium in gastroesophageal reflux disease （GERD）. Method Immunohistochemistry was used to examine the distribution and expression of Occludin in esophageal epithelium from 62 cases with GERD and 10 case with other diseases as control. Results The Occludin was normal continuous membrane expressed in 10 contrast esophageal epithelium, while in 32 nonerosive reflux disease patients, normal expression was seen in 4 cases, different degree of abnormal expression was seen in 28 cases, including incomplete membrane expression, cytoplasmic expression and expression depletion respectively, and in 30 reflux esophagitis patients were seen all abnormal expression including incomplete membrane expression, cytoplasmic expression and expression depletion respectively. There was significant difference between nonerosive reflux disease and normal contrast, reflux esophagitis and normal contrast（P 〈 0.01 ）, no difference was seen between reflux esophagitis and nonerosive reflux disease （P 〉 0.05 ）. Conclusion The number of esophageal epithelial Occludin in GERD patients diminished and abnormally distributed, interfered the integrity of epithelium mucosal barrier, which might be one of the pathogenesis of GERD, there is no difference of Occludin expression between reflux esophagitis and nonerosive reflux disease esophageal epithelium.