高强度聚焦超声所致组织坏死形式实验研究

目的：了解不同强度、不同发射方式高强度聚焦超声(high itenmty focused ultrasound，UIFU)所致组织坏死形式。方法：分别以活体猪肾和新鲜离体肾癌标本为靶目标，采用FEP-BY01型高能聚焦超声肿瘤热疗机，以不同强度、不同发射方式进行辐照，辐照后即刻对靶区组织细胞进行病理学检查和细胞台盼兰染色。结果：HIFU所致组织细胞即刻死亡有4种表现形式，即凝固性坏死、融解性坏死、裂解坏死和变性死亡，死亡形式与焦点声强和发射时间及发射方式有关。结论：临床上应尽量追求变性死亡和凝固性坏死，避免裂解坏死和融解性坏死。

Experimental Research of Tissue Necrosis by High Intensity Focused Ultrasound

Objective: To evaluate the type of tissue necrosis by different intensity and different emitting methods of HIFU. Methods: Studies on pig's kidney in vivo and fresh human renal carcinoma in vitro were performed with FEP-BY01 high intensity focused ultrasound therapier. The pathology and cell typan blue test of the target tissues were observed immediatly after HIFU. Results: There were four kinds of tissue cell necrisis including: coagulative necrosis, lysis necrosis , splitting necrosis and degeneration necrisis which corresponded with intensity of focus ultrasound, emitting time and emitting methods. Conclusions: Coagulative necrosis and/or degenerative necrosis are the clinical goal but lysis necrosis and splitting necrosis should be avoided.