Anti-oxidative Responses on Hepatic Tissue of Zebrafish (<Emphasis Type="Italic">Danio rerio</Emphasis>) in a Short Duration of Sub-lethal Concentrations of Cadmium Exposure |
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Authors: | Su Mei Wu Li-Hsin Shu Jia-Hao Liu Ching Hsein Chen |
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Institution: | 1.Department of Aquatic Biosciences,National Chiayi University,Chiayi,Taiwan, Republic of China;2.Graduate Institute of Biomedical and Biopharmaceutical Sciences,National Chiayi University,Chiayi,Taiwan, Republic of China |
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Abstract: | The aim of the present study was to identify whether the responses of oxidative stress in zebrafish liver are similar to those in mammalians upon low doses of Cd2+ exposure in short durations. Fish were exposed to 1.78 μM Cd2+ (treatment) and 0.0 μM Cd2+ (control) for 0, 1, 3, and 6 h. The reactive oxygen species (ROS) and lipid peroxidation (LPO) of hepatic tissues significantly increased after 3 and 6 h of Cd2+ exposure, respectively. Antioxidants glutathione peroxidase (gpx1a), superoxide dismutase (sod), and catalase (cat) were up regulated after 1–3 h, and metallothionein isoforms (smtB and mt2) increased after 3–6 h of Cd2+ exposure. The caspase-3 and p53 mRNA expressions significantly increased threefolds after 1 h of Cd2+ exposure. Results confirmed that oxidative stress in the hepatic tissue was induced by Cd2+ within 3 h. However, anti-oxidative functions immediately up regulated, causing cell apoptosis levels to decrease after 6 h of Cd2+ exposure. |
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