Intravenous immunoglobulin modulates the expansion and cytotoxicity of CD8+ T cells |
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Authors: | Patrick Trépanier Dominique Chabot Renée Bazin |
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Institution: | 1. Department of Research and Development, Héma‐Québec, , Québec, QC, Canada;2. Department of Biochemistry, Microbiology and Bioinformatics, Laval University, , Québec, QC, Canada |
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Abstract: | Intravenous immunoglobulin (IVIg) is successfully used in the treatment of autoimmune diseases involving self‐reactive CD8+ T cells. However, its direct influence on the cytotoxic response remains unknown. Using an antigen cross‐presentation assay and a mouse model of ovalbumin (OVA) immunization, we showed that IVIg decreases the in vitro activation, proliferation and cytokine secretion of OVA‐specific CD8+ T cells (OT‐I), as well as the in vivo generation of OVA‐specific CD8+ T cells. In addition, IVIg significantly decreases the proportion of perforin‐ and CD107a‐expressing CD8+ T cells, and inhibits the cytotoxic activity of OVA‐activated OT‐I cells. The interference of IVIg with the CD8+ T‐cell response is associated with T‐cell receptor blockade, therefore reducing the interaction between effector and target cells. A similar blockade is observed on human CD8+ T cells, suggesting that the observations reported here could apply to the IVIg‐mediated improvement of CD8+ T‐cell‐mediated autoimmune conditions in human patients. |
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Keywords: | CD8+ T cells cross‐presentation cytotoxicity immunization intravenous immunoglobulin |
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