心力衰竭模型大鼠心脏肥厚指标与心功能的关系研究

目的腹主动脉(abdominal aorta,AO)缩窄法建立大鼠慢性心力衰竭模型,比较多项指标在评价心脏肥厚中的意义及其与心功能的关系。方法Wistar大鼠80只,分为手术组和对照组,比较术后第4、8、12和16周B型脑钠肽(type-B natriuretic peptide,BNP)及心脏肥厚指标的变化。结果与对照组相比,大鼠腹主动脉缩窄术后4周,心肌细胞直径(cardiac myocardial diameter,CMD)、心脏重量指数、I型胶原浓度、BNP浓度显著升高(P<0.05)。术后8周-dp/dt降低。术后12周左室收缩末压(LVSP)升高,+dp/dt降低。术后16周上述改变更明显。BNP浓度与血流动力学和心脏肥厚指标相关性良好。结论在腹主动脉缩窄法制备的大鼠慢性心力衰竭模型中,心肌细胞肥厚指标的改变出现早于心功能指标的改变。

The Relationship between Cardiac Hypertrophy Index and Cardiac Function in Chronic Heart Failure Rats

Objective To establish rat model of chronic heart failure by partially banding abdominal aorta for different time limits and to compare the effects of different index on the evaluation of cardiac hypertrophy and heart function. Methods Eighty Wistar rats were randomly divided into two groups: aortic banding group and sham-operated group(each containing 40 rats). Brain natriuretic peptide(BNP) concentration and cardiac hypertrophic changes were investigated in different groups. Results Cardiac myocardial diameter(CMD), type I collegen concentration and BNP concentration increased from 4 weeks after aortic banding(P<0.05) compared with sham-operated group. Eight weeks after the operation, -dp/dt decreased. LVSP increased from 12 weeks after the operation, and +dp/dt decreased at the same time. Changes above became more significant 16 weeks after the operation. There was a good correlation between BNP concentration and hemodynamic and hypertrophic parameters. Conclusion In the rat model of chronic heart failure by partially banding abdominal aorta, the changes of hypertrophic index emerge before the cardiac function changes.