HPLC-UV法测定十二味丹黄软坚片中芍药苷含量

目的：建立HPLC-UV法测定十二味丹黄软坚片中芍药苷含量的方法。方法：Welch Ultimate XBC18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.1%磷酸（14∶86）,流速1.0 mL·min-1,检测波长230nm,柱温30℃。结果：在0.0103~3.0989μg范围内检测样品中芍药苷含量与峰面积线性关系良好（r=0.9998）;日内和日间精密度RSD分别为0.67%和1.71%;重复性试验（n=6）的RSD为1.67%;平均加样回收率（n=9）为93.42%,RSD为1.49%。结论：本方法测定结果可靠,重复性好,适用于十二味丹黄软坚片中芍药苷的含量测定。

Determination of Paeoniflorin in Danhuang Ruanjian Tablets by HPLC- UV

Objective：To establish an HPLC -UV method for determining the content of paeoniflorin in Danhuang Ru- anjian Tablets. Methods ：The column was Welch Uhimate XB- C18 column （4.6 mm × 150 mm,5 μm）. The mobile phase consisted of acetonitrile （A） and 0.1% phosphoric solution （B） with a ratio of 14% A to 86% B. The flow rate was 1.0 mL min-1 and the detection wavelength was set at 230 nm and the column temperature was maintained at 30 ℃. Results ： The calibration curve was linear with in the range of 0. 0103 - 3. 0989 μg for the concentration of paeoniflorin （ r = 0. 9998）. RSD of intra - day and inter - day preeisions were 0.67% and 1.71%. The reproducibility （ n = 6） RSD was 1.67%. The accuracy of reproducibility （u = 6） was 1.67%. The average recovery rate was 93.42% （ RSD = 1.49% ,n = 9 ）. Cortclusions：This method is accurate, sensitive and reproducible and it can be used to determinate the content of paeoniflorin in Danhuang Ruanjian Tablets.