电针对慢性应激抑郁模型大鼠海马内甘丙肽表达的影响 |
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引用本文: | 莫雨平,;姚海江,;宋洪涛,;许安萍,;唐银杉,;王鑫,;加吾拉·阿不力孜,;赛音朝克图,;张淑静,;李志刚. 电针对慢性应激抑郁模型大鼠海马内甘丙肽表达的影响[J]. 中医药学刊, 2014, 0(9): 2115-2117 |
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作者姓名: | 莫雨平, 姚海江, 宋洪涛, 许安萍, 唐银杉, 王鑫, 加吾拉·阿不力孜, 赛音朝克图, 张淑静, 李志刚 |
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作者单位: | [1]北京中医药大学针灸推拿学院,北京100029; [2]北京中医药大学基础医学院,北京100029; [3]内蒙古国际蒙医医院,内蒙古呼和浩特010020 |
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基金项目: | 国家自然科学基金项目(81072860) |
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摘 要: | 目的:观察电针对慢性应激抑郁模型(chronic unpredictable mild stress,CUMS)大鼠海马内甘丙肽(galanin,Gal)蛋白及mRNA表达的影响,探讨Gal在大鼠实验性抑郁症发病过程中的作用机制及电针干预对其的影响。方法:将36只清洁健康雄性SD大鼠,随机分为正常组、模型组及电针组,每组12只。孤养结合CUMS21d复制抑郁症模型,通过开野试验观察大鼠行为学变化,采用酶联免疫吸附法和实时定量荧光PCR检测Gal蛋白及mRNA的表达。结果:模型组大鼠开野实验水平运动及垂直运动明显减少(P〈0.01),电针可逆转此变化(P〈0.01);模型组大鼠海马内GAL蛋白和mRNA表达均明显下降(P〈0.01),电针可改善此病理变化(P〈0.05)。结论:慢性轻度不可预见性应激可使大鼠海马内GAL的表达量下降,电针可能通过增加海马内GAL表达发挥抗抑郁作用。
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关 键 词: | 电针 慢性应激 抑郁症 大鼠 海马 甘丙肽 |
Influence on Expression of Galanin in Hippocampus of Electro- Acupuncture Treatment for Chronic Unpredictable Mild Stress - Induced Depression Rats |
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Affiliation: | MO Yuping, YAO Haijiang , SONG Hongtao , XU Anping ,TANG Yinshan , WANG Xin , Jia Wula · A Bulizi ,Saiyin Chaoketu ,ZHANG Shujing ,LI Zhigang( 1. School of Acupuncture - Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China; 2. School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China ; 3. Inner Mongolia International Hospital of Mongolian Medicine, Hohhot 010020, Inner Mongolia, China) |
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Abstract: | Objective: To investigate the influence on the expression of galanin(Gal) protein and mRNA in the hippo- campus of rat with chronic unpredictable mild stress which had the electro -acupuncture (EA) treatment and to elucidate the possible role of Gal in the development of experimental depression and the effect of EA for it. Methods : 36 adult male SD rats were randomly divided into normal control group( n = 12) ,model group ( n = 12) and EA group ( n = 12). Depression model was established by lonely raising and chronic unpredictable mild stress for 21 days. Open - field test was performed to test the behavior of rats. The expressions of hippocampus galanin and Gal mRNA were detected by using enzyme linked immunosorbent assay (ELISA) and real- time PCR techniques respectively. Results: The cross and rear score of the model group were significantly lower than that of the control group while electro - acupuncture treatment could reverse it. The GAL protein and mRNA were significantly lower in the model group while electro - acupuncture can im- prove these pathological changes. Conclusion: Chronic unpredictable mild stress can reduce GAL expressions in rats'hip- pocampus and electro - acupuncture treatment can increase the expression of GAL to prevent depression. |
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Keywords: | electro - acupuncture chronic unpredictable mild stress depression rat hippocampus galanin |
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