榭皮素对GSNO诱导损伤的内皮细胞的保护作用及其机制研究

目的：考察榭皮素（Quercetin）对S-亚硝基化谷胱甘肽（GSNO）诱导损伤的内皮细胞的保护作用，探讨其保护机制。方法：内皮细胞（EA．hy926）预先给予不同浓度榭皮素，之后给予GSNO损伤，建立模型。采用：噻唑兰染色（MTT法）检测细胞存活率测定，之后通过对超氧化物歧化酶（SOD），丙二醛（MDA），谷胱甘肽过氧化物酶（GSH—PX），活性氧（ROS），Caspase-3等指标测定进行分析。结果：榭皮素可明显提高内皮细胞内SOD，GSH—Px的活性，抑制MDA的产生，降低ROS的活性，减少细胞凋亡率，具有统计学差异（P〈0．05）。结论：榭皮素对GSNO诱导损伤的细胞具有保护作用，其机制可能与榭皮素可提高细胞内抗氧化酶活性有关。

Research on Protective Effect of Quercetin on Endothelial Cell Injury Induced by S- Nitrosoglutathione and Its Machanism

Objective ：To investigate the protective effect of quercetin on endothelial cell injury induced by S -nitrosoglutathione, and its protective mechanism. Methods：Different concentrations of quercetin were given to endothelial cell （ EA. hy926） in advance,and then GSNO was treated to form the injury model. MTr method was used to value the cell viability. The expression of SOD, MDA, GSH - PX and ROS were analyzed. Results ： The application of quercetin significantly protected the endothelial ceils from GSNO injury. The activation of SOD and GSH - PX was increased, and the expressions of MDA were down regulated by quercetin pretreatment. The generation of ROS was also significantly decreased. Finally, results in the percentage of apoptosis was down （ P 〈 0. 05 ） . Conclusion ： Quercetin has protective effects on GSNO -induced apoptosis on endothelial cell, and the protective mechanism may enhance the antioxidant by quercetin.