| 二脱甲氧基姜黄素对体外HepG2 Caspase-3活性的影响 |
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| 引用本文： | 姚滢,黄燕芬.二脱甲氧基姜黄素对体外HepG2 Caspase-3活性的影响[J].中华中医药学刊,2014(3):493-495,I0003. |
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| 作者姓名： | 姚滢 黄燕芬 |
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| 作者单位： | 浙江中医药大学生命科学学院,浙江杭州310053 |
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| 基金项目： | 国家创新训练计划项目（201210344005）;浙江省中医药科技计划项目（2011ZB026）;浙江省大学生科技创新项目暨新苗人才计划项目（2012R410014） |
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| 摘 要： | 目的:研究二脱甲氧基姜黄素对体外培养人肝癌细胞HepG2 Caspase-3蛋白酶活性的作用。方法:MTT法检测二脱甲氧基姜黄素对体外培养人肝癌细胞HepG2的抑制作用;分光光度检测其对Caspase-3酶活性的影响;免疫组化法检测其对人肝癌细胞HepG2 Caspase-3蛋白的表达。结果:二脱甲氧基姜黄素对人肝癌细胞HepG2有明显的抑制作用(P<0.001)且呈剂量关系,与姜黄素有显著差异(P<0.001);二脱甲氧基姜黄素能增强Caspase-3酶活性,增强Caspase-3蛋白的表达。结论:二脱甲氧基姜黄素能激活Caspase-3蛋白活性,这可能也是它体外抑制肝癌细胞HepG2生长的机理之一。
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| 关 键 词： | 二脱甲氧基姜黄素 MTT 免疫组化法 Caspase-3 |
Effect of B Isdemethyoxycurcumin on Caspase-3 of HepG2 in vitro |
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| YAO Yin,HUANG Yanfen.Effect of B Isdemethyoxycurcumin on Caspase-3 of HepG2 in vitro[J].Chinese Archives of Traditional Chinese Medicine,2014(3):493-495,I0003. |
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| Authors: | YAO Yin HUANG Yanfen |
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| Institution: | (Life Science College, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang,China) |
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| Abstract: | Ojective ： To study the effects of bisdemethyoxycurcumin on the activity of Caspase - 3 in HepG2. Method： MTI＂ was used to observe its effects on anti - proliferation of HepG2. The activity of Caspase - 3 was detected by spectro- photography. The Caspase - 3 protein expression was tested by immunohistochemical method. Result ： The bisdemethyoxy- curcumin could inhibit the growth of HepG2 ceils （P 〈0.001 ） and it was more effective on anti - tumor than curcumin （P 〈 0. 001 ）. It had significant effect on increasing Caspase- 3 expression. Conclusion： The bisdemethyoxycurcumin could inhibit the growth of HepG2. It can increase the expression of protein Caspase - 3. It can increase the activity of Caspase - 3, which may be one of its mechanisms associated with the inhibition of HepG2 cells growth. |
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| Keywords: | bisdemethyoxycurcumin MTT immunohistochemical method Caspase - 3 |
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