| HPLC测定仙鹤草中山奈酚-3-O-(6-p-香豆酰基)-β-D-吡喃葡萄糖苷含量 |
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| 引用本文： | 赵艳霞,陈芳,邓雁如.HPLC测定仙鹤草中山奈酚-3-O-(6-p-香豆酰基)-β-D-吡喃葡萄糖苷含量[J].中华中医药学刊,2014(3):644-646. |
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| 作者姓名： | 赵艳霞 陈芳 邓雁如 |
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| 作者单位： | 天津中医药大学,天津市中药化学与分析重点实验室,天津300193 |
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| 摘 要： | 目的:建立高效液相色谱法测定仙鹤草药材中山奈酚-3-O-(6-p-香豆酰基)-β-D-吡喃葡萄糖苷含量的方法。方法:采用依利特ODS C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.05%磷酸(30∶70),流速为1.0 mL·min-1,柱温为30℃;检测波长315 nm。结果:山奈酚-3-O-(6-p-香豆酰基)-β-D-吡喃葡萄糖苷的线性范围为1.2728.904μg·mL-1(r=0.9999);加样回收率为100.5%,RSD为1.94%。结论:该方法简便易行、准确、重复性好,为仙鹤草药材的质量控制提供了依据。
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| 关 键 词： | 仙鹤草 山奈酚-3-O-(6-p-香豆酰基)-β-D-吡喃葡萄糖苷 HPLC |
Determination of Tiliroside in Agrimoniae Herba by HPLC |
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| ZHAO Yanxia,CHEN Fang,DENG Yanru.Determination of Tiliroside in Agrimoniae Herba by HPLC[J].Chinese Archives of Traditional Chinese Medicine,2014(3):644-646. |
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| Authors: | ZHAO Yanxia CHEN Fang DENG Yanru |
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| Institution: | (Tianjin University of Traditional Chinese Medicine, Tianjin Key Laboratory of Chinese Medicine Chemistry and Analysis, Tianjin ,300193, China) |
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| Abstract: | Objective ： To establish the HPLC method for determination of tiliroside in Agrimoniae herba. Methods ： The chromatographic conditions were Hypersil ODS C18 column （4.6 mm × 250 mm,5 μm） at 30 ℃, acetonitrile - 0.05 % phos- phoric acid as the mobile phase. The flow rate was 1.0 mL min-1, and the detection wavelength was 315 nm. Results：Linearity relationships was 1. 272 - 8. 904 μg mL-1 （ r = 0.9999 ）, the average recovery was 100.5% （ RSD = 1.94% ） Conclusion： The method is sensitive and accurate and it is available for the quality control of Agrimoniae herba. |
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| Keywords: | Agrimoniae herba tiliroside HPLC |
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