| 云南省恶性疟原虫多药抗性基因Asn86Tyr多态性调查 |
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| 引用本文： | 杨亚明,窤daguIS,高白荷. 云南省恶性疟原虫多药抗性基因Asn86Tyr多态性调查[J]. 寄生虫病与感染性疾病, 2001, 9(2): 52-54 |
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| 作者姓名： | 杨亚明 窤daguIS 高白荷 |
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| 作者单位： | 1. 云南省疟疾防治研究所,
2. Department of Medical Parasitology,London School of Hygiene and Tropical Medicine, |
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| 基金项目： | 英中学术联系项目(0992/301) |
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| 摘 要： | 目的检测云南恶性疟原虫多药抗性基因(Pfmdr1)Asn86Tyr的多态性,探索其与氯喹敏感性表现型的关系.方法用等位基因特异PCR和限制性酶切片段长度分析技术(AS-PCR/RFLP).结果在86%(19/22)的氯喹抗性分离株中检测到Pfmdr1基因的Asn86Tyr两种多态性,携带编码86Tyr多态变异的占50%,而含编码Asn86多态的也是50%.结论在云南氯喹抗性高度流行区,Pfmdr1基因Asn86Tyr的多态性是普遍存在的.
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| 关 键 词： | 恶性疟原虫 氯喹抗性 等位基因特异PCR Pfmdr1多态性 |
| 文章编号： | 1005-2534(2001)02-0052-03 |
| 修稿时间： | 2000-09-17 |
STUDY ON PLASMODIUM FALCIPARUM Pfmdr1 GENE ASSOCIATED WITH CHLOROQUINE RESISTANCE IN YUNNAN PROVINCE |
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| YANG Ya ming ,Adagu IS ,GAO Bai he. STUDY ON PLASMODIUM FALCIPARUM Pfmdr1 GENE ASSOCIATED WITH CHLOROQUINE RESISTANCE IN YUNNAN PROVINCE[J]. Parastoses and Infectious Diseases, 2001, 9(2): 52-54 |
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| Authors: | YANG Ya ming Adagu IS GAO Bai he |
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| Affiliation: | YANG Ya ming 1,Adagu IS 2,GAO Bai he 1 |
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| Abstract: | Novel allele specific PCR and RFLP have been used to 22 isolates for detecting the mutation of Pfmdr1 Asn 86 Tyr associated with chloroquine resistance in Yunnan Province so as to explore an agreement between chloroquine resistance phenotype and the point mutation. The results showed that a total of 19 (86%) isolates detected out the Pfmdr1 Asn 86 Tyr , of which 10 chloroquine resistance isolates did not show the point mutation of the Pfmdr1 86 Tyr , while 9 isolates revealed the point mutation (A to T) of the Pfmdr1 86 Tyr . The incomplete agreement found between chloroquine resistance phenotype and Pfmdr1 (86 Tyr ) mutation would support the view that other genetic factors as well as Pfmdr1 may be involved in chloroquine resistance. |
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| Keywords: | Plasmodium falciparum chloroquine resistance allele specific PCR Pfmdr1 gene Yunnan Province |
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