电针对非酒精性脂肪肝大鼠血清及肝组织白介素-18的影响

目的:探讨电针对非酒精性脂肪肝(NAFLD)大鼠白介素-18(IL-18)的影响及治疗NAFLD的机制。方法:将44只SD大鼠随机分为造模组33只,空白组11只。造模大鼠喂养高糖高脂饲料5周制备NAFLD模型,造模成功后,随机分为模型组、电针组和非穴组。电针组针刺"足三里""丰隆""三阴交""太冲"穴(连续波,频率2Hz,强度1.5V),非穴组针刺大鼠尾巴中1/3段随机4处,每日治疗1次,连续4周。采用全自动化学分析仪检测大鼠空腹血糖(FBS),放射免疫法检测空腹胰岛素(FINS),计算空腹胰岛素抵抗指数(FIRI),采用双抗体夹心酶联免疫吸附法检测血清及肝组织匀浆IL-18含量变化,HE染色观察肝组织病理学改变。结果:针刺4周后,模型组FBS、FINS、FIRI、肝组织和血清IL-18与空白组比较都显著升高(P<0.01),模型组肝组织出现不同程度的脂肪变性;电针组FBS、FINS、FIRI、肝组织匀浆IL-18和血清IL-18与模型组比较都显著下降(P<0.05),电针组肝组织内脂肪性变减轻;非穴组FBS、FINS、FIRI、肝组织匀浆IL-18和血清IL-18与模型组比较变化都不明显(P>0.05),非穴组肝组织内脂肪性变无明显改善。结论:电针可能通过降低肝组织及血清IL-18,阻断多糖攻击肝脏所致的损伤作用,改善肝脏功能,而实现对NAFLD大鼠的治疗作用。

Influence of Electroacupuncture Stimulation on Serum and Hepatic Interleukin-18 Expression in Nonalcoholic Fatty Liver Rats

Objective To observe the effect of electroacupuncture(EA) stimulation of "Zusanli"(ST 36),etc.on se-rum sugar and insulin contents,serum and liver interleukin-18(IL-18) levels in nonalcoholic fatty liver disease(NAFLD) rats,so as to study its mechanism underlying improvement of NAFLD.Methods Forty-four SD rats were randomized into normal control,model,EA and non-acupoint groups,with 11 rats in each group.EA(2 Hz,1.5 V) was applied to bilateral "Zusanli"(ST 36),"Fenglong"(ST 40),"Sanyinjiao"(ST 6) and "Taichong"(LR 3) and non-acupoint(proximal 1/3 of the tail) for 15 min,once daily for 4 weeks.IL-18 contents of serum and liver tissue were assayed by using double antibody sandwich enzyme linked immunosorbent assay(ELISA),fasting blood sugar(FBS) content was measured by enzymatic colorimetric method,and fasting insulin(FINS) level assayed by radioimmunoassay.H.E.staining of the liver tissue sections was used to display pathological changes.Results Compared with the normal control group,serum FBS and FINS contents,serum and hepatic IL-18 levels and fasting insulin resistance index(FIRI) in the model group were significantly increased(P<0.01).While compared with the model group,the levels of serum FBS,FINS,FIRI and IL-18 as well as hepatic IL-18 content in the EA group were markedly decreased(P<0.05).The fatty degeneration in hepatic tissue was relieved.No significant changes of the five indexes mentioned above were found in the non-acupoint group(P>0.05).H.E.staining of the liver tissue showed that in the model group,the hypatocyte was clearly increased in the size,with different degrees of fatty degeneration or necrosis,and inflammatory cell infiltration.These changes were relatively milder in the EA group rather than in the non-acupoint group.Conclusion EA stimulation of "Zusanli"(ST 36),"Fenglong"(ST 40),etc.can suppress NAFLD induced upregulation of blood sugar,serum insulin,insulin resistance index and serum and hepatic IL-18 levels in fatty liver rats,which may contribute to its effect in relieving polysaccharide attack induced liver damage.