电针“三阴”穴对慢性非细菌性前列腺炎大鼠细胞免疫功能的影响

目的:探讨电针"三阴"穴对慢性非细菌性前列腺炎大鼠细胞免疫功能的影响。方法:将40只雄性大鼠随机分为正常对照组、模型组、舍尼通组、电针组,每组10只。在皮内注射同种异体Wistar雄性大鼠前列腺蛋白提纯液,辅以双重免疫佐剂复制自身免疫性慢性非细菌性前列腺炎大鼠模型。电针组、舍尼通组分别给予电针"三阴"穴和舍尼通药物灌胃治疗,连续治疗2个疗程(疗程间隔1d,共15d)。称取前列腺湿重并计算前列腺指数,光镜下观察各组动物前列腺组织形态结构,利用流式细胞仪检测各组动物血浆中CD4+、CD8+的百分含量。结果:各组动物前列腺湿重及前列腺指数比较差异无统计学意义(P>0.05)。与正常对照组比较,模型组前列腺组织形态及结构受损明显,舍尼通组、电针组前列腺组织结构有不同程度恢复,但电针组更为显著。模型组血浆CD4+百分含量显著减少(P<0.01),CD4+与CD8+比值明显降低(P<0.05);与模型组比较,舍尼通组血浆CD4+百分含量明显增加(P<0.05),电针组血浆CD4+百分含量及CD4+与CD8+比值明显升高(P<0.01,P<0.05)。结论:电针"三阴"穴能有效地调节慢性非细菌性前列腺炎大鼠的细胞免疫应答反应,使全身免疫细胞CD4+数目增多,局部免疫能力增强,从而达到治疗目的。

Effect of Electroacupuncture at "Sanyin" Acupoints on Cellular Immune Function in Rats with Chronic Abacterial Prostatitis

Objective To observe the effect of electroacupuncture(EA) at "Sanyin" acupoints(bilateral "Jiayin" and "Zhongyin") on the cellular immune function of rats with chronic abacterial prostatitis(CAP),so as to explore its mechanism underlying relieving CAP.Methods Forty male Wistar rats were randomly divided into four groups: control,model,medication(Cernilton) and EA(n=10 rats/group).The CAP model was made by injection of allogeneic prostatein purification liquid + Freund's complete adjuvant(1∶1) and diphtheria,pertussis,tetanus(DPT) vaccine(0.5 mL).EA was applied to bilateral "Jia-yin" acupoints(located at the bilateral groins at the horizontal level of the upper border of the pubic symphysis) and "Zhongyin" [the midpoint between the "Huiyin"(CV 1) and the scrotum root,punctured with filiform needle] for 20 min,once daily for two therapeutic courses(15 days/course,one day break between the two courses).Rats of the medication group were given with oral administration of Cernilton(13 mg/kg) once daily for 15 days.Following killing the rats under deep anesthesia,the prostate wet weight,and prostate index(prostate weight/body weight) were determined.Pathological changes of the prostate tissue were examined by light microscope after sectioning and haematoxylin(HE) staining.Plasma levels of CD4+ Th-and CD8+ T-lymphocytes were assayed with flow cytometry.Results Compared with the control group,the morphological structure of prostate tissues of rats in the model group was severely damaged(proliferation of the prostate epithelium and inflammatory cell infilitration),which was relatively milder in the EA group.The plasma CD4+ level and CD4+/CD8+ ratio were significantly lower in the model group than in the control group(P<0.01,P<0.05).Compared with the model group,plasma CD4+ levels were significantly increased in both medication and EA groups(P<0.05,P<0.01),and the CD4+ level of the EA group was considerably higher than that of the medication group(P<0.05).The ratio of CD4+/CD8+ in the EA group was remarkably higher than that in the model group(P<0.05).No significant differences were found among the four groups in the prostate weight,prostate index and plasma CD8+ levels(P>0.05).Conclusion EA stimulation of "Sanyin" points is beneficial to relieve chronic abacterial prostatitis,which may contribute to its effect in strengthening the cellular immune function via upregulation of plasma CD4+ and CD4+/CD8+ levels.