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| 移植MMSCs改善大鼠实验性心肌梗死后心室重构 | |
| 引用本文： | 伍徐娴,何志旭,沈祥春,周中军,刘鉴.移植MMSCs改善大鼠实验性心肌梗死后心室重构[J].基础医学与临床,2010,30(1):48-53. |
| 作者姓名： | 伍徐娴 何志旭 沈祥春 周中军 刘鉴 |
| 作者单位： | 1. 贵阳医学院,法医学教研室,贵州贵阳,550004 2. 贵阳医学院,组织工程与干细胞实验中心,贵州贵阳,550004 3. 贵阳医学院,药理学教研室,贵州贵阳,550004 4. 贵阳医学院,寄生虫学教研室,贵州贵阳,550004 |
| 基金项目： | 贵州省优秀科技教育人才省长专项资金项目(黔省专合字2005-146号);;贵州省科技攻关项目(黔人领函【2005】11号文件) |
| 摘 要： | 目的探讨骨髓间充质干细胞(MMSCs)移植对大鼠实验性心肌梗死(MI)后心功能及心室重构的影响。方法开胸结扎冠状动脉左前降支(LAD)制作MI动物模型,造模成功后12～14d经舌下静脉移植已转染增强型绿色荧光蛋白(pEGFP-C1)的MMSCs,分4及8周检测动物的血流动力学、左心及全心质量指数、微血管密度测定、双盲计数法测定胶原含量,荧光显微镜观察绿色荧光的表达。结果移植组心率(HR)较同期模型组显著下降,4周时血压无明显改变,8周时则显著增高。移植组左心质量指数和全心质量指数均较同期模型组显著降低。移植组的微血管密度较同期模型组明显增高。移植组的胶原含量较同期模型组显著降低。结论移植MMSCs可改善实验性MI大鼠心功能及心室重构。 |
| 关 键 词： | 心肌梗死 骨髓间充质干细胞 心室重构 |
| 收稿时间： | 2009-2-11 |
| 修稿时间： | 2009-5-19 |
Transplantation of marrow mesenchymal stem cells improves the ventricular remodeling and heart functions to acute MI rat models |
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| WU Xu-xian,HE Zhi-xu,SHEN Xiang-chun,ZHOU Zhong-jun,LIU Jian.Transplantation of marrow mesenchymal stem cells improves the ventricular remodeling and heart functions to acute MI rat models[J].Basic Medical Sciences and Clinics,2010,30(1):48-53. | |
| Authors: | WU Xu-xian HE Zhi-xu SHEN Xiang-chun ZHOU Zhong-jun LIU Jian |
| Institution: | 1.Dept. of Forensic Medicine;2.Lab. of Tissue Engineering and Stem Cell;3.Dept. of Pharmacology;4.Dept. of Parasitology;Guiyang Medical College;Guiyang 550004;China |
| Abstract: | Objective To study the effects of marrow mesenchymal stem cells on heart functions and ventricular remodeling after myocardial infarction in rats. Methods Myocardial infarction model was established by ligation of the left anterior descending coronary artery (LAD) in adult SD rats. 4 and 8 weeks after MMSCs implantation,hemodynamic evaluations,left ventricular weight/body weight ratio and heart weight/body weight ratio were determined. HE staining was performed for counting microvasculars and Van Gieson sta... |
| Keywords: | myocardial infarction marrow mesenchymal stem cells ventricular remodeling |
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