异丙酚对大鼠肺缺血再灌注损伤保护作用的实验研究

目的:通过大鼠肺缺血再灌注模型,观察异丙酚( Propofol)对大鼠缺血再灌注损伤(ischemia reperfusion,IR)肺的保护作用,并探讨其作用机制.方法:随机将30只SPF级雄性SD大鼠分为3组:假手术组(n=10,左侧开胸后不阻断肺门)、肺缺血再灌注组(n=10,开胸后左侧肺门阻断45 min再灌注120 min)和异丙酚组(n=10,开胸后左肺门钳闭前30 min时股静脉注射异丙酚5mg·kg-1,继用微量泵以50 mg·kg-1·h-1的速度持续静脉输注,左侧肺门阻断45 min再灌注120 min).3组在开胸前均给予气管插管、机械通气.再灌注结束时抽取左心室血检测动脉血氧分压( PaO2)；获取左肺组织,光镜下观察左肺组织和细胞损伤情况,计算左肺湿干重比,评价肺水肿程度；制作肺组织匀浆并用酶联免疫吸附( ELISA)法检测其中丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量和超氧化物歧化酶(SOD)活性.结果:与假手术组比较,肺缺血再灌注组及异丙酚组大鼠肺W/D值、肺组织中的MDA、TNF-α、IL-6含量明显增高(均P＜0.05)；PaO2明显下降,SOD活性减弱明显降低(均P＜0.05).异丙酚组与缺血再灌注组比较,大鼠的PaO2升高(P＜0.05),肺组织中的MDA、TNF-α、IL-6含量及肺W/D值下降(P＜0.05),SOD活性增强(P＜0.05).肺组织病理切片显示假手术组损伤轻微,肺缺血再灌注组损伤严重,异丙酚组损伤重于假手术组但轻于肺缺血再灌注组.结论:异丙酚对缺血再灌注损伤SD大鼠的肺组织有一定的保护作用,其保护作用可能与异丙酚能清除氧自由基,抑制炎症因子产生有关.

Experimental study of Propofol protective effect on lung ischemia-reperfusion injury in rats

Objective: To observe the protective effect of Propofol on lung ischemia-reperfusion injury in rats.Methods: Thirty male SPF Sprague-Dawley rats were randomly divided into 3 groups: sham group(n=10,the left chest was opened without clamping the left hilum of lung),lung ischemia reperfusion injury group(n=10,the injury was induced by clamping the left lung hilum for 45 min followed by 120 min reperfusion),Propofol groupn=10,Propofol(5 mg·kg-1)was injected though femoral vein 30 min before clamping left lung hilum,then Propofol(50 mg·kg-1·h-1)was injected with the trace pump.Left lung hilum was clamped for 45 min and followed by 120 min reperfusion].Three groups were given tracheal intubation,mechanical ventilation before opening chest.At the end of the reperfusion,the blood sample was withdrawn from the left ventricals to test the PaO2,and the left lung was harvest.The injury of issue and cell were observed by light microscope and the pulmonary edema was evaluated through lung wet-to-dray(W/D) ratio.The content of malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) and superoxide dismutase(SOD) activity in lung tissues were measured by ELISA.Results: Compared with sham group,the W/D ratio,MDA、TNF-α、IL-6 content in lung ischemia reperfusion injury group and Propofol group were significant higher(all P<0.05),while the PaO2 and SOD activity were reduced(all P<0.05).As compared with lung ischemia reperfusion injury group,the W/D ratio,the content of MDA,TNF-α,IL-6 in Propofol group were decreased,while the PaO2 and SOD activity in Propofol group were increased notably(P<0.05).Histopathology showed that lung tissue was slightly damaged in sham group,baldly damaged in lung ischemia reperfusion injury group and the Propofol group was lighter than that in lung ischemia reperfusion injury group.Conclusions: Propofol can prevent SD rats lung ischemia reperfusion injury to some extent by removing oxygen free radicals and inhabiting inflammatory factor.