甲状腺乳头状癌中cortactin、N-WASP的表达及其与临床病理学特征的关系

背景与目的：甲状腺乳头状癌是最常见的甲状腺癌病理类型。分析侵袭性伪足关键蛋白cortactin、N-WASP在甲状腺乳头状癌组织中的表达水平及其与临床病理学特征的关系。方法：选取2015年1月—2017年12月复旦大学附属肿瘤医院病理科保存的甲状腺乳头状癌组织标本及对应癌旁组织89对，采用免疫组织化学方法，检测癌组织与癌旁组织中cortactin、N-WASP蛋白的表达情况并分析其与临床病理学特征之间的关系。结果：癌组织中cortactin和N-WASP的阳性表达率分别为57.30%和59.55%，高于癌旁组织的5.62%和7.87%，差异有统计学意义（P<0.05）。Cortactin的表达与甲状腺乳头状癌患者的年龄、性别、肿瘤大小无显著相关性（P＞0.05），但与有无淋巴结转移有关，N-WASP与肿瘤大小、有无淋巴结转移有关（P<0.05）。在有淋巴结转移的病例中，cortactin、N-WASP的阳性表达率均较高（P＜0.05）。结论：甲状腺乳头状癌组织中与侵袭性伪足形成相关的cortactin、N-WASP呈高表达，且与甲状腺乳头状癌的发病和淋巴结转移相关。Cortactin与N-WASP的表达水平呈正相关。

The expressions of cortactin and N-WASP in thyroid papillary carcinoma and their significance

Background and purpose: Papillary thyroid cancer (PTC) comprises the most part of thyroid cancer. The research aimed to study the expression levels of cortactin and N-WASP in PTC and their relationship with clinicopathological features. Methods: Tissues from 89 cases of PTC were collected at Department of Pathology in Fudan University Shanghai Cancer Center from Jan 2012 to Dec 2017. At the same time, 89 normal thyroid tissues were selected as controls. Immunohistochemistry was used to detect cortactin and N-WASP in cancer and adjacent tissues. The expression levels of cortactin and N-WASP proteins were measured, and analysis of their relationship with clinical features was performed. Results: The positive expression rates of cortactin and N-WASP in cancer tissues were 57.30% and 59.55%, respectively, which were higher than 5.62% and 7.87% in adjacent tissues (P<0.05). The expression of cortactin was not significantly associated with age, gender and tumor size in patients with PTC (P>0.05), but it was associated with lymph node metastasis. N-WASP was associated with tumor size and lymph node metastasis (P<0.05). The positive expression rates of cortactin and N-WASP were higher in patients with lymph node metastasis (P<0.05). Conclusion: Cortactin and N-WASP were highly expressed in PTC, and were associated with lymph node metastasis of PTC. Expression levels of cortactin and N-WASP were positively correlated in PTC.