lncRNA ABHD11-AS1通过调控STAT1/STAT3表达促进非小细胞肺癌细胞增殖与迁移 |
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引用本文: | 李正雄,武奋萍,邓博云,叶海茵,刘春香,杨志雄.lncRNA ABHD11-AS1通过调控STAT1/STAT3表达促进非小细胞肺癌细胞增殖与迁移[J].中国肿瘤临床,2019,46(21):1085-1090. |
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作者姓名: | 李正雄 武奋萍 邓博云 叶海茵 刘春香 杨志雄 |
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作者单位: | ①.深圳市龙华区中心医院肿瘤科(广东省深圳市 518110) |
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基金项目: | 本文课题受国家自然科学基金项目No.81871883 |
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摘 要: | 目的 探讨lncRNA ABHD11-AS1在非小细胞肺癌中的生物学功能和作用机制。 方法 选取2016年7月至2018年12月在广东医科大学附属医院行非小细胞肺癌手术的248例患者,采用χ2检验分析lncRNA ABHD11-AS1的表达与患者年龄、性别、临床病理分期、病理类型及吸烟状态的关系;采用Kaplan-Meier法分析ABHD11-AS1对非小细胞肺癌患者的预后意义;应用qRTPCR方法检测ABHD11-AS1在非小细胞肺癌组织和细胞系中的表达;通过体外功能测定评估敲低ABHD11-AS1对细胞增殖和转移的影响;通过Western blot实验探讨ABHD11-AS1在非小细胞肺癌中致癌作用的分子机制。 结果 lncRNA ABHD11-AS1高表达组较低表达组吸烟患者较少(P=0.02),分期更晚(P < 0.01);ABHD11-AS1高表达预示非小细胞肺癌患者预后不良;ABHD11-AS1在非小细胞肺癌组织和癌细胞株中高表达;敲低ABHD11-AS1的表达可抑制体外细胞增殖和迁移;敲低ABHD11-AS1表达抑制STAT1和STAT3癌蛋白的表达。 结论 ABHD11-AS1可能通过促进STAT1和STAT3癌蛋白的表达,从而增加非小细胞肺癌细胞的增殖、集落形成以及迁移和侵袭能力。
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关 键 词: | 长链非编码 RNA lncRNA ABHD11-AS1 非小细胞肺癌 增殖 侵袭 |
收稿时间: | 2019-09-01 |
LncRNA ABHD11-AS1 promotes proliferation and migration of non-small cell lung cancer cells by regulating STAT1/STAT3 expression |
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Institution: | ①.Department of Oncology, Longhua Central Hospital, Shenzhen 518110, China②.Department of Pulmonary Oncology, Affiliated Hospital of Guangdong Medical University, Shenzhen 518110, China③.Department of Radiation Oncology, The Seventh People's Hospital of Chengdu, Chengdu 100191, China |
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Abstract: | Objectives Long-chain non-coding RNA (lncRNA) plays an important role in tumorigenesis and development. At present, lncRNAs are still insufficiently studied in non-small cell lung cancer. This study focused on the biological function and mechanism of lncRNA ABHD11-AS1 in non-small cell lung cancer. Methods A total of 248 non-small cell lung cancer patients who underwent surgery in the Affiliated Hospital of Guangdong Medical University from July 2016 to December 2018 were selected. Chi-square test was used to analyze the relationship between the expression of lncRNA ABHD11-AS1 and age, sex, clinical pathological stage, pathological type and smoking status. Kaplan-Meier method was used to analyze the prognostic significance of ABHD11-AS1 in patients with non-small cell lung cancer. qRT-PCR was used to detect the expression of ABHD11-AS1 in non-small cell lung cancer tissues and cell lines. The effect of knockdown of ABHD11-AS1 on cell proliferation and metastasis was evaluated by in vitro functional assay. Western blot was used to investigate the molecular mechanism of ABHD11-AS1 in non-small cell lung cancer. Results LncRNA ABHD11-AS1 high expression group was related to advanced stage (P < 0.01) and had fewer smoking patients than lower expression group (P=0.02). ABHD11-AS1 high expression was predictive of poor prognosis in patients with non-small cell lung cancer. ABHD11-AS1 is highly expressed in non-small cell lung cancer tissues and cancer cell lines. Knockdown of ABHD11-AS1 expression can inhibit cell proliferation and metastasis in vitro. Knockdown of ABHD11-AS1 expression inhibits STAT1 and STAT3 oncoproteins expression. Conclusions ABHD11-AS1 may promote the proliferation, colony formation, migration and invasion of non-small cell lung cancer cells by promoting the expression of STAT1 and STAT3. |
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