肝细胞癌患者癌组织miRNA-30c水平变化及其临床意义探讨*

目的 探讨肝细胞癌（HCC）患者癌组织微小RNA（miRNA）-30c（miRNA-30c）水平变化及其与预后的关系。方法 2010年2月~2012年11月我院收治的HCC患者35例,经外科手术切除肿瘤获得癌组织标本。采用RT-PCR法检测癌组织miRNA-30c相对水平。不同临床和病理因素患者癌组织miRNA-30c水平高低的风险比（OR）和95%可信区间（CI）采用Logistic二项回归分析。采用Kaplan-Meier最小乘积法评价患者的生存率,不同miRNA-30c水平患者生存率的差异比较采用Log-Rank检验。结果 35例HCC患者癌组织miRNA-30c相对水平为0.6,95%CI为0.3~0.6,其中癌组织miRNA-30c相对水平低于0.6者13例,大于0.6者22例;不同性别、年龄、肿瘤大小、分化程度、TNM分期、有无肝炎病史和血清甲胎蛋白（AFP）水平高低患者癌组织miRNA-30c水平差异无统计学意义（P>0.05）,但17例存在淋巴结转移患者癌组织miRNA-30c水平为（0.2±0.0）,显著低于18例无淋巴结转移患者【（0.8±0.1）,P<0.05】;调整后的风险比（OR）=5.4,95%CI：1.2~20.1;癌组织miRNA-30c高水平患者总生存期为（14.5±6.7）个月,95%CI为11.0~28.4个月,显著长于低水平患者【（7.9±1.5）个月,95%CI为3.2~10.7个月,P<0.05】。结论 本研究结果表明,HCC患者癌组织miRNA-30c相对水平与患者预后有关,其是否可作为潜在的PLC诊断和预后判断的生物学标志物还需进一步研究。

Cancerous miRNA-30c levels affecting the survival of patients with hepatocellular carcinoma after hepatectomy

Objective To investigate the relationship between cancerous miRNA-30c levels and the survival of patients with hepatocellular caicinoma （HCC） after hepatectomy. Methods Thirty-five patients with HCC in our hospital between February 2010 and November 2012 were included in this study. All patients were confirmed by pathological examination after hepatectomy,and cancerous miRNA-30c levels were assayed by real-time quantitative polymerase chain reaction. Cancerous miRNA-30c levels were compared between patients with different clinical and pathological features. The survival rate of patients was evaluated by the Kaplan-Meier minimum product method,and the difference of survival rate was evaluated by Log-Rank test. Results The relative level of cancerous miRNA-30c in the 35 patients with HCC was 0.6, with 95% CI of 0.3 to 0.6,and there were 13 patientss with low cancerous miRNA-30c level less than 0.6 and 22 patients with high cancerous miRNA-30c levels greater than 0.6;there were no significant differences of cancerous miRNA-30c levels between patients with diffenrent gender,age,tumor size,degree of differentiation,TNM staging,history of viral hepatitis,and serum AFP levels（P>0.05）,however,the cancerous miRNA-30c levels in 17 patients with lymph node metastasis was（0.2±0.0）,significantly lower than 【（0.8±0.1）,P<0.05】 in 18 patients without lymph node matastasis;the adjusted risk ratio （OR）=5.4,and 95% CI:1.2 to 20.1;the total survival in patients with high cancerous miRNA-30c levels was （14.5±6.7） m,with 95%CI of 11.0-28.4 m,significantly longer than【（7.9±1.5） m,with 95%CI of 3.2-10.7 m,P<0.05】 in patients with lower cancerous miRNA-30c levels. Conclusion Our findings indicate that the cancerous miRNA-30c level is related to the prognosis of patients with hepatocellular carcinoma,and it might be a new potential biomarker for predicting the survival of patients with hepatocellular carcinoma after hepatectomy.