束缚应激模型在凉茶“降火”研究中的应用

目的 评价束缚应激模型在凉茶“降火”研究中应用的科学性及适用性。方法 急性和慢性应激束缚模型实验,均为雄性Balb/c小鼠40只,随机分为对照组、束缚组、低、中、高3个剂量凉茶组[0.8、2.4、7.2 g/（kg BW）凉茶干膏粉],每组8只。急性应激束缚模型为给药5 d,第5天给药1 h后除对照组外束缚2 h;慢性应激束缚模型为每天给药1 h后除对照组外束缚2 h,共10 d 。给予不同时间的处理后,观察小鼠一般行为,记录终体重及肝脏重量,计算肝体比,检测血清中谷丙转氨酶、谷草转氨酶及尿素氮的含量,测量肝脏中丙二醛、一氧化氮、超氧化物歧化酶水平及总抗氧化能力。结果 急性和慢性应激束缚模型,血清中谷丙转氨酶、谷草转氨酶及尿素氮含量较对照组均升高（P<0.05或P<0.01）;慢性束缚应激模型肝脏丙二醛、一氧化氮水平较对照组升高,超氧化物歧化酶水平及总抗氧化能力较对照组降低 （P<0.01）。高剂量凉茶组与慢性束缚组相比,血清中谷丙转氨酶、谷草转氨酶及尿素氮含量均降低（P<0.01）;肝脏丙二醛、一氧化氮水平降低,超氧化物歧化酶水平及总抗氧化能力升高（P<0.05或P<0.01）。结论 模拟“上火”,急性和慢性应激束缚模型均成立,且用于评价凉茶“降火”功效具有科学性和可行性。

Application of restraint stress model in studying “Jiang huo” efficacy of herbal tea

Objective To evaluate the scientificality and applicability of the restraint stress （RS） model in the study of “Jiang huo” （reducing internal heat） efficacy of herbal tea. Methods Forty male Balb/c mice were randomly divided into control group, RS model group and herbal tea （HT） groups in three doses [0.8, 2.4, 7.2g/（kg BW）, in form of dry paste powder], 8 mice in each group for the acute and chronic RS model experiments. The test substances were administrated for 5 consecutive days in the acute RS model; one hour after the last administration, all mice except control group were physically restrained for 2 hours. The test substances were administrated daily; one hour after administration, all mice except control group were physically restrained for 2 hours for 10 consecutive days in the chronic RS model. After treatment for different times, the general behavior of mice was observed, the body weight and liver weight were recorded, the ratio of liver to body was calculated, serum levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, and blood urea nitrogen （BUN） were detected, and the levels of malondialdehyde （MDA）, nitric oxide （NO）, superoxide dismutase （SOD） and total antioxidant capacity （T-AOC） in liver were determined. Results Compared with the control group, contents of serum ALT, AST and BUN were increased in both acute and chronic RS models （P<0.05 or P<0.01）, the levels of MDA and NO in liver were increased and the SOD and T-AOC levels in liver were decreased significantly （P<0.01 for all） in chronic RS model. Compared with the chronic RS model group, the contents of ALT, AST and BUN decreased （P<0.01 for all）; the levels of MDA and NO in liver was decreased, while the levels of SOD and T-AOC in liver were increased in the high dose HT group （P<0.05 or P<0.01）. Conclusion Simulated “Shang huo” （suffering from excessive internal heat）, both acute and chronic RS models were established, and they were scientific and feasible to evaluate the “Jiang huo” efficacy of herbal tea.