CHRDL1 调节结直肠癌增殖、 迁移和侵袭

目的 探讨腱蛋白样蛋白 1 (chordin-like 1, CHRDL1) 对结直肠癌 (colorectal cancer, CRC) 进 展的调节作用。 方法 数据库分析 CHRDL1 与 CRC 进展、 预后关系, 构建 CHRDL1 过表达 SW480、 HT29 细胞系并检测细胞增殖、 迁移和侵袭, 体外移植瘤实验检测肿瘤生长, 分析 CHRDL1 过表达对 CRC 细胞迁 移和侵袭作用机制。 结果 CHRDL1 在 CRC 进展中呈高表达且可影响预后。 CHRDL1 过表达可促进细胞增 殖、 迁移、 侵袭和移植瘤生长, 促进 Vimentin、 N-Cadherin、 snail 表达, 抑制 E-Cadherin 表达, CHRDL1 与 snail 存在互作关系, 干扰 snail 表达可逆转上述指标趋势。 结论 CHRDL1 可促进 CRC 细胞增殖, 还可通 过 Snail 促进 CRC 细胞上皮间质转化来发挥促进细胞迁移、 侵袭作用。

CHRDL1 Regulate the Proliferation,Migration and Invasion of Colorectal Cance

Objective To explore the effect of chordin-like1 (CHRDL1) on the progression of colorectal cancer ( CRC). Methods The relationship between CHRDL1 and CRC progression, prognosis was analyzed by the database. SW480 and HT29 cell lines with CHRDL1 overexpression were constructed. The cell proliferation, migration and invasion were assayed. The tumor growth was detected byin vitrotumor transplantation assay. The mechanism of CHRDL1 overexpression on migration and invasion of CRC cells was analyzed. Results The expression of CHRDL1 was up-regulated in CRC progression and could affect the prognosis. The overexpression of CHRDL1 could promote cell proliferation, migration, invasion and growth of the transplanted tumors. Its overexpression could also promote the enhanced expressions of Vimentin, N-Cadherin and snail, and inhibit the expression of E-Cadherin. There was an interaction between CHRDL1 and snail. Interfering with snail siRNA could reverse the trend of the above indexes. Conclusion CHRDL1 can promote the proliferation of CRC cells, it can also promote the epithelial mesenchymal transformation of CRC cells and the cell migration and invasion by Snail.