Effects of P limitation and molecules from peanut root exudates on pqqE gene expression and pqq promoter activity in the phosphate-solubilizing strain Serratia sp. S119 |
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| Affiliation: | 1. Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal 3, 5800 Rio Cuarto, Córdoba, Argentina;2. Loewe Center for Synthetic Microbiology, Philipps-University Marburg, Hans-Meerwein-Str. 6, 35043 Marburg, Germany;3. California Institute for Quantitative Biosciences, University of California Berkeley, Berkeley, California 94720;4. Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado 8020;5. Centre for Biomedical Research, Faculty of Sciences and Technology, University of the Algarve, 8005-139 Faro, Portugal;6. Department of Chemistry, University of California Berkeley, Berkeley, California 94720;1. Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082, Hunan, PR China;2. Hunan Academy of Forestry, Changsha, 410004, Hunan, PR China;3. National Engineering Research Center for Oil-tea Camellia, Changsha, 410004, Hunan, PR China;4. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, Hunan, PR China |
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| Abstract: | The mineral phosphate-solubilizing phenotype in bacteria is attributed predominantly to secretion of gluconic acid produced by oxidation of glucose by the glucose dehydrogenase enzyme and its cofactor, pyrroloquinoline quinone. This study analyzes pqqE gene expression and pqq promoter activity in the native phosphate-solubilizing bacterium Serratia sp S119 growing under P-limitation, and in the presence of root exudates obtained from peanut plants, also growing under P-limitation. Results indicated that Serratia sp. S119 contains a pqq operon composed of six genes (pqqA,B,C,D,E,F) and two promoters, one upstream of pqqA and other between pqqA and pqqB. PqqE gene expression and pqq promoter activity increased under P-limiting growth conditions and not under N-deficient conditions. In the plant–bacteria interaction assay, the activity of the bacterial pqq promoter region varied depending on the concentration and type of root exudates and on the bacterial growth phase. Root exudates from peanut plants growing under P-available and P-limiting conditions showed differences in their composition. It is concluded from this study that the response of Serratia sp. S119 to phosphorus limitation involves an increase in expression of pqq genes, and that molecules exuded by peanut roots modify expression of these phosphate-solubilizing bacterial genes during plant–bacteria interactions. |
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| Keywords: | Pyrroloquinoline quinone (PQQ) cofactor Phosphorus Peanut root exudates |
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