儿童复发性腮腺炎免疫功能检测和分析

目的:通过分析比较儿童复发性腮腺炎（juvenile recurrent parotitis,JRP）患儿与健康儿童的免疫球蛋白和T细胞亚类,探讨JRP患儿体液免疫和细胞免疫的特点,为进一步明确病因及未来免疫疗法治疗JRP提供依据。方法:回顾性分析2014年12月—2018年10月间就诊于上海交通大学医学院附属第九人民医院口腔外科的100例JRP患儿与100例健康儿童的临床资料。所有患儿均接受完善的专科检查,包括唾液腺临床检查、间歇期血清免疫学检查以及必要的影像学检查。为每一例确诊为JRP的患儿建立临床数据档案,记录患儿的一般情况、既往病史、临床症状、持续时间、血液免疫学检测和影像学检查结果。采用SPSS 23.0软件包进行统计学分析,2组间平均数的比较采用独立样本t检验。结果:JRP患儿中,低龄组和学龄组在既往发病次数、平均持续时间以及家族史方面无统计学差异。低龄组中,JRP患儿血清IgG和IgM浓度显著高于健康对照组（P<0.05）。不同年龄组中,JRP患儿血清IgE和IgA浓度均显著高于健康对照组（P<0.01）,且随年龄增长有增高的趋势;同时,JRP患儿CD4表达水平显著低于健康对照组（P<0.01）,而CD8表达水平显著高于健康对照组（P<0.01）。结论:JRP患儿的体液免疫和细胞免疫功能异常以及可能存在的超敏反应过程,可能是JRP发生、发展的原因之一。

Detection and analysis of immune function in children with juvenile recurrent parotitis

PURPOSE: Juvenile recurrent parotitis(JRP) is defined as recurrent parotid inflammation occurring in children, usually associated with non-obstructive sialectasis of the parotid gland. The etiology of JRP remains unclear, probably immunologically mediated. The purpose of the present study was to investigate the relationship between JRP and immune function based on the measurement of immunoglobulins and T-lymphocyte subset of JRP patients. METHODS: We retrospectively studied the clinical data of 100 JRP children and 100 healthy controls. All the subjects underwent clinical examination of salivary glands as well as immune function detection during the intermission of JRP and necessary radiological examination. We established a database recording demographic and clinical characteristics, medical history, immune function results, etc for each subject. SPSS 23.0 software package was used for statistical analysis. Comparison of means between two groups was performed with independent samples Student's t test. RESULTS: Serum IgG and IgM levels were significantly higher in JRP children than in normal children between 2-6 years (P<0.01). Serum IgE and IgA levels were significantly higher in JRP children than in normal children in all age strata (P<0.01), and tended to increase with age. Serum IgM levers were significantly higher in JRP children than in normal children aged<6 (P<0.05). CD4+ levels were higher in normal children than in JRP children in all age strata (P<0.01). CD8+ levels were higher in JRP children than in normal children in all age strata(P<0.01). CONCLUSIONS: Immune function derangement and anaphylaxis may be involved in the process of JRP.