颈脊髓切断对内毒素休克大鼠全身炎症反应及预后的影响

目的 研究颈脊髓切断对内毒素休克大鼠全身炎症反应及预后的影响.方法 92只SD大鼠被随机分为正常对照组(n=8)、内毒素休克模型组(n=42)及内毒素休克+颈脊髓切断组(n=42),后两组各取10只大鼠于制模后观察48 h存活率,其余大鼠再按制模后3、6、12和48 h分为4个亚组,每亚组8只动物.切断大鼠C7脊髓制备颈脊髓切断动物模型;腹腔注射内毒素脂多糖(LPS)10 mg/kg造成动物内毒素休克.处死动物开胸取血,采用高效液相色谱法(HPLC)测定血浆中去甲肾上腺素(NE)含量,用双抗体夹心酶联免疫吸附法(ELISA)检测血浆中白细胞介素-10(IL-10)及IL-6的含量.结果 模型组血浆NE含量逐渐升高,而切断组逐渐下降.但均较对照组显著升高(P均＜0.05);自6 h起切断组显著低于模型组(P均＜0.05).模型组血浆IL-10含量于3 h和6 h时显著低于对照组(P均＜0.05),之后逐渐升高(P均＞0.05);切断组于各时间点显著高于模型组,且于12 h和48 h显著高于对照组(P均＜0.05).模型组各时间点血浆IL-6含量均高于对照组(P均＜0.05);切断组3、6和12 h显著低于模型组(P均＜0.05),且与对照组差异无统计学意义,而在48 h时较对照组显著升高(P＜0.05).切断组大鼠48 h存活率高于模型组(70%比20%).结论 颈脊髓切断可增加内毒素休克大鼠血浆中IL-10的含量,抑制IL-6生成,并能提高48 h存活率,对内毒素休克大鼠的全身炎症反应及预后具有改善的作用.

Effects of cervical chordotomy on systemic Inflammatory response and the outcome in rats with endotoxlc shock induced by lipopolysaccharide

Objective To investigate the effects of cervical chordotomy on systemic inflammatory response and the outcome in rats with endotoxemia induced by lipopolysaccharide(LPS).Methods Ninety-two Sprague Dawley(SD)rats were randomly divided into three groups:normal control group (group I,n=8),endotoxemia group(group I n=42)and endotoxemia with cervical chordotomy (group Ⅱ,n=42).Endotoxemia was induced by intra-peritoneal injection of LPS 10 mg/kg.In group Ⅱ,"cervical chordotomy"was attained by transection of spinsl cord at C7 immediately before intra-peritoneal LPS administration.Ten rats of group Ⅱ and Ⅱ each were observed for 48-hour survival.The other rats were further divided into four subgroups of 8 animals each,according to the time when the animals were sacrificed.The animals were sacrificed at 3,6,12,and 48 hours after intra-peritoneal LPS injection.Heart blood samples were obtained for determination of plasma concentration of norepinephrineNE,by high performance liquid chromatography(HPLC)] and plasma concentration of interleukin-10(IL-10)and IL-6 by enzyme linked immunosorbent assay(ELISA)].Results Plasma NE concentration were significantly increased after intra-peritoneal LPS injection in group Ⅱ and Ⅱ as compared with group I and were significantly lower in group Ⅱ than in group Ⅱ starting from 6 hours after LPS(all P＜0.05).Plasma IL-10 concentration was significantly lower at 3 hours and 6 hours while plasma IL-6 concentration was significantly higher after LPS challenge in group Ⅱ than in group I at all time points(all P＜0.05).High transection of spinal cord significantly elevated plasma IL-10 level at 12 hours and 48 hours,lowered IL-6 release at 3,6,and 12 hours(all P＜0.05),and improved 48-hour survival(20%vs.70%)in group Ⅱ as compared with group Ⅱ.Conclusion Transection of spinal cord at C7 level can ameliorate the systemic inflammatory response induced by endotoxemia thus improving the outcome through elevation in IL-10 level,decreases in IL-6 release,and improves 48-hour survival.This might be attributable to loss of sympathetic nerve function.