羟基喜树碱联合足叶乙甙对肺腺癌细胞的放射增敏作用

目的：探讨拓扑异构酶（TOP0）Ⅰ抑制剂－羟基喜树碱（HCPT）与TOPOⅡ抑制剂足叶乙甙（VP-16）对肺腺癌细胞毒作用及放疗增敏作用。方法：MTT法检测HCPT、VP-16及二药在间隔不同时间应用对肺腺癌GLC-82的细胞毒作用及放疗增敏作用。结果：HCPT、VP-16单药及联合用药各实验组均对GLC-82细胞生长产生抑制作用，以HCPT＋VP-16（12h后）组对癌细胞的抑制作用最强（38．31％）。放疗后应用HCPT、VP-16可降低癌细胞存活率，其中以放疗＋HCPT＋VP-16组存活率最低。结论：HCPT与VP-16联合应用时抗癌效应呈时问依赖性，以间隔12h用药最佳。HCPT联合VP-16对肺腺癌放疗起增敏作用。

RADIOSENSITIZING EFFECT OF HYDROXYCAMPTOTHECIN COMBINED WITH ETOPOSIDE ON HUMAN PULMONARY ADENOCARCINOMA

Objective To investigate the experimental therapeutic effect of combination of hydroxycamptothecin(HCPT)with etoposide(VP-16)on human pulmonary adenocarcinoma and to study the radiosensitizing effect.Methods Cytotoxicity and radiosensitizing effect of HCPT alone,VP-16 alone,and combination of HCPT with VP-16 in different courses on human pulmonary adenocarcinoma GLC-82 cells were measured by MTT assay.Results HCPT alone and VP-16 alone had potent cytotoxicity to GLC-82 cells.The group of highest inhibition growth rate was to use VP-16 12 hours after HCPT.The group of lowest survival rate was radiation of HCPT combined with VP-16.Conclusion HCPT and VP-16 have been shown radiosensitizing effect in vitro on human pulmonary adenocarcinoma.