奥曲肽对人胃癌BGC-823细胞增殖和凋亡影响的研究

目的:研究生长抑素类似物奥曲肽(octreotide,OCT)对胃癌BGC-823细胞增殖、凋亡、细胞周期的影响及其可能的作用机制.方法:不同浓度的OCT作用于体外培养的人胃癌BGC-823细胞,以5-FU作为阳性对照,应用MTT法分析细胞增殖的抑制作用,流式细胞仪测定细胞周期分布和凋亡率,AO-EB染色观察细胞形态变化.结果:OCT对BGC-823细胞的生长、增殖产生抑制作用,该抑制作用具有量效、时效关系和饱和性;OCT作用后细胞呈典型的凋亡形态学改变,流式细胞仪检测的细胞凋亡率和AO-EB染色测得细胞凋亡率与对照组相比较差异有显著性(P<0.05);BGC-823细胞经OCT作用后,G1期细胞数明显增加,S细胞数明显减少,细胞被阻滞于G1/S 期.结论:10-5-10-3g/L 浓度的OCT能够抑制胃癌BGC-823细胞增殖,机制可能与其诱导肿瘤细胞的凋亡和出现G1/S期阻滞有关.

Effect of octreotide on cells proliferation and apoptosis in human gastric cancer cell line BGC-823

Objective:To study the effect of octreotide(OCT) on proliferation,apoptosis and cell cycle in human gastric cancer cell line BGC-823 in vitro,and further to explore the possible mechanism of inhibition. Methods: Human gastric cancer cell line BGC-823 was treated with Octreotide.5-FU was used as positive control.MTT assay and AO-EB fluorescent staining as well as flow cytometry were performed in this study. Results: Octreotide inhibited the growth and proliferation of BGC-823 in vitro within certain concentrations.The suppression was dose-dependent,time-dependent,and had staturability.Morphological variations of apoptosis were observed after treated with OCT,the apoptotic rate measured by AO-EB fluorescent staining and flow cytometry had statistical significance compared with negative group(P<005).G1phase rate gradually increased,S phase rate gradually decreased,cell cycle was arrested at G1phase in human gastric cancer cell line BGC-823 treated by OCT. Conclusion: Octreotide can inhibit the proliferation of human gastric cancer cell line BGC-823 in vitro.It might be related with inducing apoptosis and arresting cell cycle at G1/S phase.