柔红霉素在HL—60／ADR细胞内的异常分析

目的 了解化疗药物在非Ｐｇｐ耐药细胞朱ＨＬ６０／ＡＤＲ细胞内的异常分布与耐药的关系。方法 采用共聚焦激光扫描显微镜观察、荧光染色、ＭＴＴ、逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）等方法研究柔红霉素（ＤＮＲ）在ＨＬ－６０／ＡＤＲ细胞内的分布及秘耐药的关系，以及维拉帕米、布雷非尔得菌素、氯喹对细胞内ＤＮＲ异常分布的影响。结果 与ＤＮＲ在ＨＬ－６０细胞核、乐均匀分布不同，在ＨＬ６０／ＡＤＲ，ＤＮＲ呈点棘状分

Altered subcellular distribution of daunorubicin in the non P-glycoprotein mediated multidrug resistant cell line HL-60/ADR

Objective To investigate DNR subcellular distribution in the non P glycoprotein mediated multidrug resistant cell line HL 60/ADR and its relation to multidrug resistance. Methods DNR subcellular disposition was studied by confocal scanning laser microscopy, fluorescent methods, MTT and RT PCR. The effects of verapamil, brefeldin A, chloroquine were also examined. Results In the drug sensitive cell line HL 60 DNR fluorscence distributed evenly in the nucleus and cytoplasm, while in the resistant cell line DNR distributed in a punctate pattern in the cytoplasm and was reduced in the nucleus. Verapamil, brefeldin A, but not chloroquine could recover the intracellular distribution of DNR from punctate to even in the resistant cell line. Conclusion Altered subcellular disposition of DNR in resistant cell line was involved in the mechanism of multidrug resistance.