| 高脂饲料加速阿霉素肾小球硬化模型的建立 |
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| 作者姓名： | 陈继红 孙伟 高坤 周栋 刘丽 赖仁胜 朱萱萱 |
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| 作者单位： | [1]南京中医药大学第一临床医学院, 江苏南京210046 [2]南京中医药大学附属医院, 江苏南京210029 |
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| 摘 要： | 目的建立局灶节段性肾小球硬化动物模型。方法对SD大鼠行右肾摘除术，分2次尾静脉注射阿霉素，每次3mg／kg，并喂饲高脂饲料。结果该模型表现为浮肿、大量蛋白尿、低蛋白血症、高脂血症；病理学检查、计算机图像分析及免疫组织化学染色显示，肾小球主要表现为系膜基质增加，肾小球出现局灶节段性硬化，细胞外基质FN、CoLⅣ表达上调。结论本模型为研究慢性肾小球疾病的机制及治疗创造了条件。
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| 关 键 词： | 肾小球硬化 动物模型 阿霉素 高脂饲料 大鼠 高脂饲料 阿霉素 慢性肾小球疾病 硬化模型 Models Adriamycin 条件 创造 治疗 机制 研究 表达上调 细胞外基质 局灶节段性硬化 系膜基质 染色显示 免疫组织化学 图像分析 计算机 检查 |
| 文章编号： | 1000-5005（2007）03-0161-03 |
| 收稿时间： | 2007-01-05 |
| 修稿时间： | 2007-03-06 |
Establishment of Adriamycin Glomerule Sclerosis Models Assisted by High-lipid Fodder |
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| Authors: | CHEN Ji-hong SUN Wei GAO Kun ZHOU Dong LIU Li LAI Ren-sheng ZHU Xuan-xuan |
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| Institution: | 1. First Clinical College of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu, 210046, China;2. Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing , Jiangsu , 210029, China |
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| Abstract: | OBJECTIVE To establish animal models for focal segmental glomerulosclerosis.METHOD Nephrectomy of the right kidney of SD rats was performed,followed by caudal vein injection of adriamycin twice(3mg/kg each time) and feeding of high lipid fodder.RESULT This model is manifested by edema, large amount of albuminuria,hypoproteinemia and hyperlipemia.Pathological examinations,computer image analysis and immunohistochemical staining suggested that the glomerule was manifested by the increase of the mesenterium base,focal segmental glomerulosclerosis,and an increase in the expression of extracellular matrix FN and Col IV.CONCLUSION This model provides the necessary conditions for the study of the pathogenesis of chronic diseases of the glomerule. |
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| Keywords: | glomemloscelerosis animal model adriamycin high lipid fodder rat |
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