土鳖虫对血虚小鼠红细胞免疫功能的实验研究

目的：研究土鳖虫对环磷酰胺致小鼠“血虚”模型红细胞免疫功能的影响，为探讨“虚-瘀”及活血化瘀机理提供新思路和途径。方法：腹腔注射环磷酰胺0．01g/kg建立小鼠“血虚”动物模型，观察土鳖虫对实验动物红细胞免疫粘附功能指标的影响。采用酶联免疫吸附法(ELISA)测血清抗心磷脂抗体(ACA)，ACA-IgG、ACA-IgA和ACA-IgM含量。造模前后称体重。解剖分离心、肝、脾、肺、肾、胸腺各脏器称重，计算脏器系数。给正常小鼠ig土鳖虫，观察其对血清锌、钙水平的影响。结果：环磷酰胺(0．01g/kg)ip小鼠，随着“血虚”模型的形成，小鼠的红细胞C3b受体花环率(RBC-C3bRR)下降,血清抗心磷脂抗体(ACA)ACAIgG、ACAIgA含量明显升高，体重下降及脾脏，胸腺重量减轻。土鳖虫(25g/kg)ig能提高“血虚”小鼠RBC-C3bRR,降低血清ACAIgG、ACAIgA水平，能纠正环磷酰胺引起的体重下降及增加脾脏及胸腺免疫器官的重量，土鳖虫(25g，kg)ig正常小鼠可升高血清锌、钙的水平。结论：土鳖虫通过提高红细胞CR1活性，增加红细胞免疫粘附功能，抑制血清ACA水平,血清中锌、钙含量的升高，可作为药物“生物药效探针”。

Experimental study of Eupolyphaga Sinensis Walker on red blood cell immunity in mice with blood-deficiency

Objective:To study the effect of Eupolyphaga Sinensis Walker (ESW) on red blood cell immune adherence (RCIA) in mice model with blood-deficiency by Cyclophosphamide , aimingatfurther research on the mechanism of blood stasis syndrome and promoting blood circulation and removing blood stasis.Methods:This model was made in mice by i.p. cyclophosphamide (0.01 g/kg).It has been observed that how the influence of ESW on the function of red cell immune adherence. The serum anticardiolipin antibodies (ACA),ACA-IgG?ACA-IgA and ACA-IgM levels were measured by using enzymelinked immunosorbent assay (ELISA). The mice body weight were weighted before model and after model and glandular weight were weighted. The serum levels of trace element zinc calcium were determined in healthy mice.Results:The results showed there is a decline in garland rate of red blood cell C3b receptors (RBC-C3bRR) in mice when animal model of Blood - deficiency by cyclophosphamide (0.01 g/kg) form, the serum ACA-IgG and ACA-IgA levels were markedly elevated,while mice body weight and spleen thymus weight markedly depressed. ESW (25 g/kg) increased RBC-C3bRR in the mice model with Blood-deficiency,restified the mice model body weight loss by cyclophosphamide, increased spleen thymus weight .ESW (25 g/kg) increased serum zinc calcium level in the normal mice.Conclusion:ESW increases activity of CR 1 of red blood cells and RCIA, inhibits serum ACA-IgG and ACA-IgA.ESW enhances serum zinc calcium level, that can be a good biological pharmacodynamics.