| HPLC法测定松针中二氢槲皮素含量 |
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| 引用本文： | 樊铁波,刘红煜,汤青,刘树民. HPLC法测定松针中二氢槲皮素含量[J]. 中国药师, 2009, 12(8): 1046-1048 |
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| 作者姓名： | 樊铁波 刘红煜 汤青 刘树民 |
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| 作者单位： | [1]绥化学院化学与制药工程系,黑龙江绥化 [2]黑龙江生物科技职业学院,黑龙江绥化 [3]黑龙江中医药大学中医药研究院,黑龙江哈尔滨 |
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| 摘 要： | 目的：建立松针中二氢槲皮素测定方法。方法：采用HPLC法测定松针中二氢槲皮素的含量，采用Kromasil C18色谱柱（250×4．6mm 5μm）；以甲醇-水（50：50）为流动相，流速：1．0ml·min^-1；柱温：30℃；检测波长320nm。结果：线性范围：0．25—1．25μg，r=0．9998。平均回收率为100．4％，RSD1．3％（n=6）。结论：方法可用于测定松针中二氢槲皮素。
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| 关 键 词： | 松针 二氢槲皮素 高效液相色谱 含量测定 |
Determination the Content of Dihydroquercetin in Pine Needles by HPLC |
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| Affiliation: | Fan Tiebo , Liiu Hongyu, Tang Qing, Liu Shumin ( 1. Department of Chemical and Pharmaceutical Engineering, Siuhua College, HeilongJiang, Sinhua 152061, China ;2. College of Heilongjiang Biotechndogy Vocational ; 3. Heilongjiang University of TCM , Harbin ) |
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| Abstract: | Objective： To establish a mothod for determiniation the content of dihydroquercetin in pine needles. Method： HPLC was adopted, Kromasil C18 column（ 250mm × 4.6mm ,5μm） was used, the mobile phase was methanol-water（ 50：50 ） , the flow rate was 1.0ml · min^-1 , the column temperature was 30℃ ,the detection wavelength was 320nm. Result： The linear range of dihydroquercetin was 0.25 -1.25μg （ r = 0. 999 8 ）. the average recorery was 104.4%, RSD was 1.3% （ n = 6 ）. Conclusion： The method can be used for delerm;nation the content of dihy, droquercetin in pine needles. |
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| Keywords: | Pine needles Dihydroquereetin HPLC Content determination |
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