| 调肝理脾方抗酒精性肝纤维化大鼠的免疫组化及图像分析研究 |
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| 引用本文： | 赵丽云,丁霞,蒙一纯,贲长恩,田德禄.调肝理脾方抗酒精性肝纤维化大鼠的免疫组化及图像分析研究[J].北京中医药大学学报,2000,23(2). |
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| 作者姓名： | 赵丽云 丁霞 蒙一纯 贲长恩 田德禄 |
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| 作者单位： | 1. 北京中医药大学组织胚胎学教研室,北京,100029
2. 北京中医药大学东直门医院,北京,100700 |
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| 基金项目： | 国家中医药管理局资助课题! (No.97A201) |
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| 摘 要： | 肝纤维化的形成主要是细胞外基质 (ECM)的过度产生和沉积所致 ,肝脏星形细胞 (HSC)的活化和增殖是多数ECM的细胞来源 ,抑制HSC的活化和增殖是治疗的关键。通过应用免疫组织化学染色的方法 ,观察了调肝理脾方对实验性酒精性肝纤维化大鼠肝脏HSC形态及功能的影响 ,并通过图像分析及统计学处理 ,验证了药物抗酒精性肝纤维化的作用机理和作用效果。结果提示调肝理脾方是通过抑制HSC的活化和增殖 ,从而起到抑制ECM合成的作用。这也是调肝理脾方预防和治疗酒精性肝纤维化的机理所在
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| 关 键 词： | 肝脏星形细胞 酒精性肝纤维化 免疫组化 调肝理脾 结蛋白 图像分析 |
Immunohistochemistry and Image Analysis on the Anti-alcoholic Liver Fibrosis Function of Tiaoganlipifang In Rat |
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| Zhao Liyun.Immunohistochemistry and Image Analysis on the Anti-alcoholic Liver Fibrosis Function of Tiaoganlipifang In Rat[J].Journal of Beijing University of Traditional Chinese Medicine,2000,23(2). |
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| Authors: | Zhao Liyun |
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| Abstract: | The liver fibrosis is mainly induced by the overproduction and deposit of extracellular matrix (ECM). And the activation and proliferation of hepatic stellate cells (HSC) are main sources of the ECM, inhibiting the activation and proliferation of HSC is the key point of the treatment. Through the method of immunohistochemical staining, the effect of Tiaoganlipifang on the rat with experimental alcoholic liver fibrosis was observed and the action mechanism was testified through the image analysis and statistical treatment. The results suggested that Tiaoganlipifang was functionized with inhibiting the synthesis of ECM through inhibiting the activation and proliferation of HSC. |
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| Keywords: | Hepatic stellate cells Alcoholic liver fibrosis Immunohistochemistry: Regulating the function between the liver and the spleen Image analysis |
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