乳腺癌组织中 miR-21的表达及意义

目的 对乳腺癌组织及其相应癌旁组织中miR-21进行定量检测,分析其表达与临床病理特征的及意义.方法 采用茎环实时荧光逆转录聚合酶链反应(RT-PCR)检测60例乳腺癌及其对应癌旁组织中miR-21的表达量,分析其表达与肿瘤大小、TNM分期、组织学类型、淋巴结转移、雌激素受体(ER)、孕激素受体(PR)等临床病理特征的关系.结果 实时RT-PCR方法 检测miR-21表达的敏感性和特异性良好.miR-21在乳腺癌组织中的表达明显高于癌旁组织(P＜0.001);miR-21的高表达还与较高的TNM分期,淋巴结转移及PR的表达程度有关(各分组间P＜0.01及＜0.05);miR-21的表达在"三阴性乳腺癌"(ER,PR,CerbB-2均为阴性)和"非三阴性乳腺癌"之间的表达存在统计学差异(P=0.029).结论 miR-21在乳腺癌组织中高表达,其水平可能与乳腺癌的恶性程度有关.

Expression of miR-21 in breast carcinoma and its significance

Objective:To examine the level of expression of microRNA-21(miR-21) in breast cancer and its adjacent non-cancerous tissues, and analyze the miR-21 expression with the clinicopathological factors of breast cancer.
Methods:Specimens of breast cancer and adjacent tissues were collected from 60 patients. Their miR-21 expression was detected by Stem-loop real-time RT-PCR, and levels of miR-21 expression in tumor size, TNM clinical stage, histology, lymph node involvement, and hormone receptor status were compared.
Results:High sensitivity, specificity and precision of Stem-loop real-time RT-PCR allowed for direct analysis of expression of miR-21 in breast cancer tissues and adjacent tissues. The expression of miR-21 in breast cancer tissues was markedly higher than in adjancent tissue （P＜0.001). Elevated miR-21 expression was also positively correlated with advanced TNM stage, lymph node metastasis, and low PR levels（P=0.007,0.001,and 0.012 respectively）. Moreover, the levels of expression of miR-21 in triple-negative breast cancer（TNBC）were higher than those in non-TNBC types（P=0.029）.
Conclusions:High levels of expression of miR-21 are correlated with degree of malignancy of breast cancer.