新型钩端螺旋体的毒力调查

目的　对从安徽省金钱蛙肾中分离出的新血清群———顾群秦型人代表株EG79的毒力进行实验调查。方法　将生长状况良好的EG79钩端螺旋体注入豚鼠体内,观察豚鼠的活力、体温、体重的改变,七天后抽取心血做MAT和培养。将其解剖后取肾脏、肝脏做培养及PCR检查。结果　感染了EG79的豚鼠的活力下降,有发热、颤抖的现象;体重减轻、体温升高;病理解剖发现肝、肺有明显病变;PCR检测为阳性。分析EG79对豚鼠有一定的致病力,至于是否对人也有同样的致病能力,尚需要做进一步的实验以证明。

Study on the virulence of a new serovar of Leptospira interrogans

The virulence of a new type of Leptospira interrogens, the representive strain of Gu serogroup, Qin serovar EG79, isolated from Ronan plancyi was investigated in the present study. The well-grown culture of the EG79 strain was injected into guinea pigs to observe the alterations of viability ,body temperature and body weight. Seven days later, blood samples were taken from heart to do MAT and microbial cultivation. At the same time, the kidney and spleen tissues taken from autopsy were undergone microbial cultivation, pathological examination and PCR assay. The result showed that the viabilities of the infected guinea pig declined with rise of body temperature and reduction of body weight. Definite pathological lesions could be demonstrated in the liver and lungs. The PCR showed a positive result. It is concluded that this new type of Leptospira interrogens possesses a certain degree of virulence to guinea pigs, but further experimentations are necessary to prove its virulence to human beings.