| 抗人膀胱癌噬菌体单链抗体的初步制备 |
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| 引用本文： | 张宏斌,王元利.抗人膀胱癌噬菌体单链抗体的初步制备[J].免疫学杂志,1997,13(2):119-121,127. |
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| 作者姓名： | 张宏斌 王元利 |
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| 作者单位： | [1]广州军区广州总医院医学实验科 [2]广州军区广州总医院泌尿外科 |
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| 摘 要： | 应用ＰＣＲ方法，从杂交瘤细胞中扩增鼠抗人膀胱癌抗体重、轻链可变区基因，经ＤＮＡ接头连接后与噬菌粒ｐＣＡＮＴＡＢ５重组，转化大肠杆菌后制备噬菌体抗体文库，通过亲和筛选、再转染、克隆化及鉴定，得到高亲和力抗人膀胱癌噬菌体单链抗体。
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| 关 键 词： | 噬菌体抗体 制备 单克隆抗体 膀胱肿瘤 |
PREPARATION OF THE PHAGE SINGLE CHAIN ANTIBODY OF ANTI HUMAN BLADDER CANCER |
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| Zhang Hongbin,Jiang Yuehua,Wang Jie,Yang Taicheng,Wang Yuanli,Tan Shangheng,Yu Maosheng.PREPARATION OF THE PHAGE SINGLE CHAIN ANTIBODY OF ANTI HUMAN BLADDER CANCER[J].Immunological Journal,1997,13(2):119-121,127. |
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| Authors: | Zhang Hongbin Jiang Yuehua Wang Jie Yang Taicheng Wang Yuanli Tan Shangheng Yu Maosheng |
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| Abstract: | Using the polymerase chain reaction,immunoglobulin variable region genes were amplified from bladder cancer hybridomas The heavy and light chain DNA products were assembled into a single gene using a DNA linker The anti bladder cancer single chain DNA fragment was ligated into a phagemid vector pCANTAB5,and the recombinant vector was subsequently introduced into E Coli TG1 cells for construction of phage display libraries of anti human bladder cancer By phage rescue and affinity selection,the anti bladder cancer phage single chain antibody of defined specificity and affinity was obtained |
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| Keywords: | Phage antibody Bladder cancer Preparation |
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