凝胶色谱法同时测定香菇多糖制剂中多糖分子量和含量

目的 建立一种同时测定香菇多糖制剂中多糖分子量分布和含量的凝胶色谱方法.方法 采用TSKgel G5000PWxl、G4000PWxl、G3000PWxl （7.8 mm×30 cm,7μm）凝胶色谱柱串联,以右旋糖苷为对照品,0.1 mol· L-1乙酸钠-乙酸水溶液为流动相,通过示差检测器检测,柱温为60℃,流速为0.6 mL·min-1,进样量为100 μ上L.结果 Dextrans的分子量对数logM/W与峰保留时间在22～47 min内呈良好的线性关系（r=0.9990）；多糖进样量在20.12～201.20 μg范围内与峰面积呈良好的线性关系（r =0.9999）,加样回收率平均值为106.33％,RSD=1.55％（n=9）.结论 本方法简便、准确、重复性好,可用于香菇多糖注射制剂的质量控制.

Determination of Lentinan Molecular Weight and Its Content in Lentinan Injection Preparation by GPC

Objective To establish a method for the determination of lentinan molecular weight and its content in lentinan Injection preparation by Gel Permeation Chromatography （GPC）. Methods TSKgel GS000PWxl, G4000PWxl and G3000PWxl columns （7.8 ram）〈 30 cm, 7 fire） were used in series. Dextran was used as reference substance. The mobile phase was 0.1 mol . L1 CHa COONa-CHa COOH solution. The detector was RID. The column temperature was set at 60℃. The flow rate was 0. 6 mL .min1 , and the inject volume was 100 ffL. Results The logM/W of the molecular weight presented a good linearity with the peak retention time of Dextran in range of 22 47 min, r = 0. 9990. The progress of lentinan presented a good linearity with the peak square in range of 20. 12 201.20 fig, r=0. 9999. The average recovery of lentinan content was 106.33% with RSD--1.55% （n =9）. Conclusion The established method is simple, rapid, and accurate, which can be used for the quality control of lentinan injection preparation.