| 人胃癌p16/CDKN2基因蛋白质水平的异常表达 |
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| 引用本文： | 宋震亚, 徐荣臻, 钱可大, 唐训球, 赵小英, 林敏.人胃癌p16/CDKN2基因蛋白质水平的异常表达[J].世界华人消化杂志,1997,5(3):139-140. |
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| 作者姓名： | 宋震亚 徐荣臻 钱可大 唐训球 赵小英 林敏 |
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| 作者单位： | 310009,浙江省杭州市,浙江医科大学附属二院. |
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| 摘 要： | 目的ｐ１６／ＣＤＫＮ２基因是新近发现的肿瘤抑制基因，已有研究表明该基因在许多肿瘤出现缺失、突变或重排．胃癌细胞系中有高频率纯合子缺失，应用Ｓｏｕｔｈｅｒｎｂｌｏｔ和ＳＳＣＰ技术在胃癌手术标本中未发现有该基因的缺失．本文首次研究胃癌中ｐ１６／ＣＤＫＮ２基因在蛋白水平的表达．方法应用蛋白Ａ金探针免疫组化技术对３０例胃癌和２０例正常胃粘膜标本的ｐ１６进行分析．结果所有正常对照组均有ｐ１６表达，其阳性细胞百分率为７２５５％±１７２２％；而胃癌中的阳性细胞百分率为５４９０％±２８９０％，明显低于正常对照（Ｐ＜００５）．在肿瘤病例中有２３３３％（７／３０）ｐ１６表达减低，１０％（３／３０）未表达ｐ１６．此外有１例呈过度表达．结论胃癌中有ｐ１６的异常表达，免疫组化技术可能较准确地反映ｐ１６的表达，更适合于临床研究．
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| 关 键 词： | 胃肿瘤 p16／CDKN2基因 异常表达 |
Department of Gastroenterology, 2nd Affiliated Hospital, Zhejiang Medical University, Hangzhou 310009, China |
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| Abstract: | AIM To investigate for the first time p16/CDKN2 gene expression at protein level in human gastric cancer. p16/CDKN2 gene is a newly identified tumor suppresser gene and has been shown to be deleted, mutated, or rearranged in numerous human tumors. Recently, homozygous deletion of the p16/CDKN2 gene has been reported in gastric cancer cell lines with high frequency,but none in surgical specimens of gastric adenocarcinomas by Southern blot and SSCP analysis. |
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| Keywords: | Stomach neoplasms p16/CDKN2 gene |
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