冬凌草甲素诱导多药耐药细胞系K562／A02凋亡,逆转耐药性？…

目的：探讨冬凌草甲素诱导多药耐药细胞系Ｋ５６２／Ａ０２凋亡、逆转耐药性的作用。方法：以不同浓度的冬凌草甲素处理Ｋ５６２／Ａ０２及敏感株Ｋ５６２／Ｓ细胞，流式细胞仪检测细胞凋亡率，四甲基偶氮唑盐（ＭＴＴ）比色法检测半数抑制率（ＩＣ５０），分析冬凌草甲素对两种细胞柔红霉素（ＤＮＲ）、高三尖杉酯碱（ＨＨＴ）敏感性的影响，流式细胞仪检测冬凌草甲素对两种细胞内ＤＮＲ浓度的影响。结果：冬凌草甲素可诱导两种细胞

EFFECTS OF ORIDONIN ON INDUCING THE MULTI-DRUG RESISTANT CELL LINE K562/A02 TO APOPTOSIS AND REVERSING THEIR MULTI-DRUG RESISTANCE

Objective:To study the effects of oridonin on inducing the multi drug resistant cell line K562/A02 to apoptosis and reversing their multi drug resistance.Method:K562/A02 and sensitive K562/S cells were treated with oridonin on a variety of concentration.The apoptosis rates of the two cell lines were detected by flow cytometry,IC 50 values of the two chemotherapy drugs (DNR and HHT) were analyzed by MTT assay to analyze the reversal effects;DNR concentration in the cells were detected by flow cytometry.Result:Oridonin can induce the two kinds of cells to apoptosis and the apoptosis rates of the two cell lines were not significantly different,the apoptosis rates were increased with the increasing of dose or time,IC 50 values of DNR and HHT of K562/A02 cells were reduced significantly,and DNR concentration in K562/A02 cells was upregulated.As to K562/S cells,however,this compound couldn't affect them.Conclusion:In K562/A02 cells,oridonin can induce cell apoptosis,reverse multi drug resistance,so this compound is a kind of very promising anti leukemia traditional chinese drug.