转染VEGF-ASODN对胃癌细胞Survivin表达的影响

目的了解转染VEGF—ASODN（血管内皮生长因子反义寡核苷酸）对胃癌细胞Survivin（生存素）表达、细胞生长的作用．方法人工合成硫代磷酸化VEGF-ASODN，转染胃癌细胞SGC-7901，用Western-blot法检测细胞Survivin蛋白表达量、用流式细胞仪检测转染后细胞凋亡情况、用MTT检测转染后细胞生存力、细胞生长曲线检测转染后细胞生长情况．结果转染VEGF-ASODN能降低细胞Survivin蛋白，与对照组比较差异有统计学意义（P〈0．05），转染后细胞凋亡与对照组比较差异有统计学意义（P〈0．05），能降低细胞的生存力及抑制细胞生长．结论VEGF-ASODN能抑制Survivin蛋白表达、抑制胃癌细胞生长、增加胃癌细胞的凋亡．

Effects of VEGF Antisense oligonucleotide on Expression of Survivin in Gastric Cancer Cells

Objective To observe the effects of VEGF antisense oligonucleotide on expression of survivin and growth in gastric cancer cells. Methods The VEGF-ASODN was synthesised artificially. After transfected with VEGF-ASODN, survivin protein was measured by Western-blot, apoptosis was detected by FCM, survival of cells was detected by MTI＇. Growth of cells was evaluated by growth curve. Results Transfected with VEGF-ASODN, it reduced the expression of survivin remarkably, increased apoptosis and decreased the survival and the growth of the cells. Conclusion VEGF-ASODN can reduce the expression of survivin, suppress the growth and increase the apoptosis of gastric cancer cell.