ERCCl、BRCAl在维族、汉族非小细胞肺癌患者中的表达分析

目的：研究探讨维吾尔族和汉族非小细胞肺癌组织中DNA修复家族成员核苷酸切除修复交叉互补组1（excision repair cross complementation groupl，ERCCl）及乳腺癌易感基因（breast cancer susceptibility genel，BRCAl）的表达及其对化疗疗效的预测价值。方法：收集2009年1月-2012年6月经病理证实的初治晚期NSCLC标本200例．其中维吾尔族患者和汉族患者分别为94例和106例，采用免覆组化方法检测ERCCl、BRCAl的表达情况。分析二者表达的特点和相关性。结果：维吾尔族NSCLC组织中ERCCl表达阳性率为31．9％，汉族NSCLC组织中ERCCl表达阳性率为30．2％，两组ERCCl的表达在差异无统计学意义（χ^2=0．069，P〉0．05）；维吾尔族NSCLC组织中BRCAl表达阳性率为80．9％，汉族NSCLC组织中BRCAl表达阳性率为59．4％，两组BRCAl的表达差异有统计学意义（χ^2=10．781，P〈0．05）：在维吾尔族或汉族NSCLC组织中，ERCCl和BRCAl表达差异有统计学意义（χ^2=5．726，P〈0．05；χ^2=6．726，P〈0．05），且表达均存在显著负相关（r=-0．247，P〈0．05；rs=-0．252，P〈0．05）。结论：维吾尔族与汉族NSCLC组织中BRCAl表达存在差异，且ERCCl和BRCAl表达存在负在关。ERCCl和BRCAI可为临床上维吾尔民族非小细胞肺癌患者个体化治疗及判断预后提供参考。

Differential expression of excision repair cross complementation group 1 and breast cancer susceptibility gene 1 in Uygur and Han patients with non-small cell lung cancer

Objective：To investigate the differential expression of excision repair cross complementation group 1 （ ERCC1 ） and breast cancer susceptibility gene 1 （ BRCA1 ） and their prognostic value to chemotherapy in Uygur and Han patients with non-small cell lung cancer （NSCLC）. Methods： We sampled the specimens of 200 （94 Uygur and 106 Han） patients with pathology-diagnosed advanced NSCLC who were admitted between January 2009 and June 2012. Immunohistochemistry assay was employed to detect the ERCC1 and BRCA1 expression for subsequent analysis on their characteristics and correlation. Results： The difference in the positive rate of ERCC1 in NSCLC tissues between Uygur and Han patients were unremarkable （31.9% vs. 30.2% , χ^2= 0. 069, P 〉 0.05 ）. Uygur patients yielded a significantly higher positive rate of BRCA1 expression in NSCLC tissue than Han patients （80.9% vs. 59.4% , χ^2= 10. 781, P 〈0.05）. The expression of ERCC1 differed substantially from that of BRCA1 in Uygur （χ^2= 5. 726, P 〈 0.05 ） or Hart patients （χ^2=6. 726, P 〈 0.05 ）. A negative correlation was found between ERCC1 and BRCA1 expression in Uygur（rs = - 0. 247, P 〈 0.05 ） and Han patients （ rs= - 0. 252, P 〈 0.05 ）. Conclusion ： The fact that Uygur and Han patients with NSCLC differ substantially in BRCA1 expression and that ERCC1 expression correlates negatively with BRCA1 in NSCLC tissues may shed light on implementation of individualized therapy and judgment of prognosis of chemotherapy in Uygur patients.