甲钴胺联合葛根素对糖尿病大鼠神经病变的保护作用

目的：观察甲钻胺联合葛根素用药对糖尿病大鼠神经病变的保护作用及机制。方法：腹腔注射链脲佐菌素诱导糖尿病大鼠模型，随机分为模型组、甲钻胺组、葛根素组、甲钻胺葛根素联合组，并设置空白对照组。连续给药8周，监测大鼠空腹血糖及糖化血红蛋白；组织切片观察坐骨神经病理变化；并采用生化法检测大鼠坐骨神经组织超氧化物歧化酶（SOD）以及丙二醛（MDA）水平。结果：葛根素组及两药联合组大鼠血糖及糖化血红蛋白与模型组比较明显降低（P〈0．01）；与模型组相比甲钻胺及甲钴胺葛根素联合组可升高SOD活性，减少MDA生成（P〈0．05）。结论：甲钴胺、葛根素对DPN均有一定的保护作用，且以联合用药组作用最显著。其机制可能与升高坐骨神经组织SOD活性，减少氧化应激损伤有关。

The protection effect of puerarin and mecobalamine against on diabetic peripheral neuropathy rats

Objective： To investigate the effect of mecobalamine and puerarin on diabetic peripheral neuropathy rats and the mechanism. Methods： Rats were divided into control group, model group, Puerarin group, Mecobalamine group, and Puerarin plus Mecobalamine group. The pathological changes of sciatic nerve were observed, the blood glucose and lycosylated hemoglobin were determined,and SOD and MDA of the sciatic nerve tissue were detected by biochemical process and ELISA. Results： Compared with the model group,the blood glucose and glycosylated Hb were Significantly decreased（P〈 0.01）, SOD and MDA raised and reduced respecti- vely in the Mecobalamine group and Puerarin plus Mecobalamine group （/9 〈 0.05）. Conclusion： Both drugs have protective effect on diabetic peripheral neuropathy rats,but their combination is more effective. The mechanism may be related to the improvement of the activity of SOD and the decrease of oxidative stress injury.