人胎儿膀胱发育中肥大细胞的组织化学研究

目的：研究人胎儿膀胱的肥大细胞的组织化学性质的变化。材料和方法：取４３例不同胎龄的人胎儿膀胱切片做ＨＥ染色和ＡＢ－Ｓ染色并测定肥大细胞的临界电解质浓度值偏低，其颗粒的糖胺多糖的硫酸化和度偏低；６月龄后肥大细胞数量则明显增多，颗料经ＴＢ染色呈深紫红色，ＡＢ－Ｓ染色出现红色出现红色及红蓝混合色，临界电解质浓度值偏高，颗粒的糖的硫酸化程度出偏高，可含肝素。结论：在人胎儿膀胱发育中肥大细胞的组化性质发生了

Histochemical Study on Mast Cell of Human Fetus in the Developing Bladder

To study the histochemical property changes of the mast cells in bladder of human fetus. Materials and Methods: The sections of different months old fetuses were stained respectively with Toluidine blue(TB) and Alcian blue-Safranin(AB-S) mixture. The critical electrolyte concentration (CEC) in the mast cells and their fluorescent characteristic of Berberine sulphate staining were also investigated. Results: In fetuses younger than 6 months, the number of mast cells was less and the granules were pale violet and blue with TB and AB-S stainning respectivey, the concentration of CEC was also lower and their granular glycosaminoglycan had a lower degree of sulphation. However, in fetuses older than 6 months the number of mast cells increased greatly, the granules stained with TB showed deep violet color and red or mixed red-blue in AB-S mixture. The results showed a positive relationship between critical electrolyte concentration and high sulphation of granular glycosaminoglycan. Conculsion: Significant changes takes place in the histochemical properties of the mast cells during the development of the bladders of human fetus.