胡椒醇提物的药理学实验研究

目的 探讨胡椒醇提物局部外涂的药理作用.方法 分别采用二甲苯致小鼠耳廓肿胀炎症模型和磷酸组胺致豚鼠足部瘙痒模型测试胡椒70%乙醇提取液的局部抗炎止痒作用;利用图像分析系统测定小鼠耳廓局部应用胡椒70%乙醇提取液前与应用后5,10,20 min及腹腔注射肾上腺素后5,10,15,20 min耳廓细动、静脉口径和细静脉血流态,由此评价其对小鼠耳廓正常微循环和肾上腺素引起的微循环障碍的作用.结果 胡椒70%乙醇提取液局部外涂能显著抑制二甲苯所致小鼠耳廓肿胀;显著提高豚鼠足部磷酸组胺致痒阈;显著增加正常小鼠细动、静脉口径及细静脉血流速度,并能明显对抗肾上腺素引起的细动、静脉收缩和细静脉血流速度减慢.结论 胡椒70%乙醇提取液局部外用具抗炎、止痒和改善微循环作用.

Experimental Study of Alcohol Extract from Pepper on Pharmacology

Objective To investigate the pharmacological effect of alcohol extract from pepper on topical application. Methods Two experimental models including xylene - induced ear edema in mice and histamine phosphate induced - foot pruritus in guinea pigs were employed to evaluate topically anti-inflammatory and antipruritic action of 70% alcohol extract from pepper. Internal diameters of auric arterioles and venules in mice were measured initially and then 5,10 and 20min after application of 70% alcohol extract from pepper and again 5,10,15 and 20 min after ip Adrenaline with image analysis system. Simultaneously blood flow state in auric venules was observed. Results Topical administration of 70% alcohol extract from pepper significantly inhibited ear edema induced by xylene, significantly increased threshold quantity of histamine phosphate with which elicited pruritus in guinea pigs, significantly dilated auric arterioles and venules in mice, speeded up blood flow rate of venules. Furthermore, it inhibited adrenaline - induced contraction of arterioles and venules as well as the reduction of blood flow rate of venules. Conclusion 70% alcohol extract of pepper has pharmacological activities of anti-inflammation, alleviating itching and improving micro-circulation.