马桑内酯点燃癫(癎)大鼠海马及齿状回区CGRP变化的初步研究

目的了解马桑内酯(CL)点燃癫大鼠海马及齿状回降钙素基因相关肽(CGRP)表达变化,探讨其可能的作用及机理。方法健康雄性SD大鼠30只,随机分为2组:对照组(C组,n=6)和实验组(n=24)。实验组肌肉注射CL0.20ml/kg,次/60h,平均20次;24h内有3~5级(Racinescale)性发作,或连续2次注射期间发作10次或以上为点燃(K组),否则为未点燃(NK组);C组注射等量生理盐水。取各组脑组织行CGRP免疫组化染色,应用Image-proplus4.5图像分析系统检测其变化。结果11只大鼠点燃成功,K组CGRP表达较NK组和C组显著增强(P<0.05)。结论CL点燃癫诱发大鼠海马及齿状回CGRP表达明显增强,CGRP可能在CL点燃癫中发挥一定的作用。

The preliminary study on change of Calcitonin gene-related peptide (CGRP) in hippocampus and dentate gyrus of the kindled epilepsy models of rats induced by Coriaria Lactone(CL)

Objective To study the different changes of Calcitonin gene-related peptide(CGRP) in hippocampus and dentate gyrus of the kindled epilepsia rat models induced by Coriaria Lactone(CL), and to explore the possible effect and action mechanism of CGRP in pathogenesis of epilepsy. Methods 30 healthy male Sprague-Dawley(SD) rats were randomized into test group (n=24) and control group (C Group, n=6). The test group received repeated CL intramuscular injection at subthreshold dosage (0.2 ml/kg) until the rats were kindled, and the control group received normal saline (NS). These injections were repeated every 60 hours. After average injection for 20 times, the rats whose seizures reached 3~5 grade (Racine scale) within 24 hours after CL injection and had 10 or more seizures between 2 injections were thought as the kindled group (K Group). The rest were thought as the non-kindled group(NK Group). The brain tissue of all groups (K group, NK group and C group) were dissected and collected in order to perform immunohistochemically stain, and then the results were examined and analyzed semi-quantitively for expression of CGRP by means of Image-pro plus4.5 digital micro-image analysis systems. Results (1) 11 rats were kindled. (2) CGRP of the kindled rats in hippocampus and dentate gyrus increased significantly as compared with that of the non-kindled rats and the control rats(P<0.05). Conclusions CGRP of the kindled rats in hippocampus and dentate gyrus increased significantly, and CGRP maybe play an important role in epilepsy.