翼状胬肉中MMP-2,TIMP-2和TGF-β1的表达意义

目的:探讨MMP-2及其抑制剂TIMP-2和影响它们作用的TGF-β1在翼状胬肉中的表达及意义。方法:采用Maxvision免疫组化技术检测在20例翼状胬肉和10例正常球结膜中MMP-2、TIMP-2及TGF-β1的表达,并比较两组中表达的差异。结果:MMP-2和TGF-β1在翼状胬肉中的表达的阳性率显著高于正常球结膜中它们的阳性表达率(z=-4.618,-4.376,P<0.01),而TIMP-2在两组中的表达差异无显著性(z=-1.319,P>0.05)。经Spearman等级相关检验分析,在翼状胬肉中,MMP-2和TGF-β1的阳性表达率存在正相关(r=0·686,P<0.01);而MMP-2和TGF-β1与TIMP-2阳性表达率之间无相关(r=-0.410,-0.143,P>0.05)。结论:TGF-β1介导的MMP-2和TIMP-2表达失衡在翼状胬肉的发生、发展及侵犯角膜过程中发挥着重要的作用。

Expression and significance of MMP-2,TIMP-2 and TGF-β1 in pterygium

AIM: To investigate the expression and significance of matrix metalloproteinase-2 (MMP-2), tissue inhibitor of metalloproteinase-2 (TIMP-2) and transforming growth factor beta (TGF-β1) in the pterygium.·METHODS: Maxvision immunohistochemical staining was used to detect the expression of MMP-2,TIMP-2 and TGF-β1 in 20 cases with pterygium and 10 cases with normal conjunctiva.·RESULTS: There was significant difference of expression of MMP-2 and TGF-β1 between the pterygium and the normal conjunctiva (z=-4.618,-4.376; P<0.01). But there was no statistic significance for TIMP-2 expression (z=-1.319; P>0.05). By the Spearman rank correlation test analysis, there was correlation between the expression of MMP-2 and TGF-β1 (r=0.686;P<0.01), and there was no correlation between the expression of MMP-2 and TIMP-2 or between the expression of TGF-β1 and TIMP-2 (r=-0.410, -0.143;P>0.05).·CONCLUSION: The imbalance induced by TGF-β1 between the expression of MMP-2 and TIMP-2 may play a critical role during the occurrence, development and invasion of pterygium.