c-myc对大鼠黄体细胞人绒毛膜促性腺激素诱导的孕酮和雌二醇生成的影响

应用离体细胞体外孵育法研究了反义ｃ ｍｙｃ寡脱氧核苷酸（反义ｃ ｍｙｃＯＤＮ）对大鼠黄体细胞人绒毛膜促性腺激素（ｈＣＧ）诱导的孕酮（Ｐ）和雌二醇（Ｅ２）生成的影响及其与外源性ｃＡＭＰ和Ｃａ２＋的关系。结果发现，反义ｃ ｍｙｃＯＤＮ能呈剂量相关方式抑制黄体细胞ｈＣＧ诱导的Ｐ和Ｅ２的生成。在浓度分别为１０和５μｍｏｌ／Ｌ时的抑制作用即具有显著意义（Ｐ＜０．０５）；而无义ｔａｔ寡脱氧核苷酸则无此作用。反义ｃ ｍｙｃＯＤＮ对黄体细胞ｈＣＧ诱导的Ｐ和Ｅ２产生的抑制作用能被加入１０－４ｍｏｌ／Ｌ二丁酰ｃＡＭＰ逆转，钙离子通道阻断剂维拉帕米对此种抑制作用具有协同效应。结果提示，ｃ ｍｙｃ癌基因参与黄体细胞ｈＣＧ诱导的Ｐ和Ｅ２生成的调控。

Effect of c-myc on the human chorionic gonadotropin induced progesterone and estradiol production of rat luteal cells

Objective: The effect of c myc antisense oligode oxynucleotides (antisense c myc ODN) on the human chorionic gonadotropin (hCG) induced progesterone and estradiol production of isolated rat luteal cells and their relationship with cAMP and Ca2+ were investigated.Design: Labortory studies.Setting: Labortory in Department of Physiology, Jiangxi Medical College.Methods: Incubation of isolated rat luteal cells in vitro.Results: Antisense c myc ODN inhibited the hCG induced progesterone and estradiol production in a dose dependent manner, at the concentration of 10 μmol/L and 5 μmol/L respectively, the inhibitions were significant(P<0.05) when compared with the control. But nonsense tat ODN had no similar effect on luteal cells. Futher experiments showed that the inhibition of antisense c myc ODN on the hCG induced progesterone and estradiol production could be reversed by dbcAMP (10-4mol/L). Verapamil (10-6mol/L) had synergistic effect on the inhibitory function of antisense c myc ODN.Conclusion: It is possible that cAMP. Ca2+ dependent pathway may promote expression of the protooncogenes, which, in turn, may lead to regulation of luteal cell function.