Calreticulin mutation analysis in non-mutated Janus kinase 2 essential thrombocythemia patients in Chiang Mai University: analysis of three methods and clinical correlations |
| |
Authors: | Ekarat Rattarittamrong Adisak Tantiworawit Noppamas Kumpunya Ornkamon Wongtagan Ratchanoo Tongphung Arunee Phusua |
| |
Affiliation: | 1. Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang-Mai University, Chiang Mai, Thailandekarat.r@cmu.ac.th ekarat_r@hotmail.com;3. Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Chiang-Mai University, Chiang Mai, Thailand;4. Central Laboratory Section, Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand;5. Division of Hematology and Oncology, Department of Pediatrics, Faculty of Medicine, Chiang-Mai University, Chiang Mai, Thailand |
| |
Abstract: | ABSTRACTObjectives: The primary objective was to determine the prevalence of calreticulin (CALR) mutation in patients with non-JAK2V617F mutated essential thrombocythemia (ET). The secondary objectives were to evaluate the accuracy of CALR mutation analysis by high-resolution melting (HRM) analysis and real-time polymerase chain reaction (PCR) compared with DNA sequencing and to compare clinical characteristics of CALR mutated and JAK2V617F mutated ET.Methods: This was a prospective cohort study involving ET patients registered at Chiang Mai University in the period September 2015–September 2017 who were aged more than 2 years, and did not harbor JAK2V617F mutation. The presence of CALR mutation was established by DNA sequencing, HRM, and real-time PCR for type 1 and type 2 mutation. Clinical data were compared with that from ET patients with mutated JAK2V617F.Results: Twenty-eight patients were enrolled onto the study. CALR mutations were found in 10 patients (35.7%). Three patients had type 1 mutation, 5 patients had type 2 mutation, 1 patient had type 18 mutation, and 1 patients had novel mutations (c.1093 C–G, c.1098_1131 del, c.1135 G–A). HRM could differentiate between the types of mutation in complete agreement with DNA sequencing. Patients with a CALR mutation showed a significantly greater male predominance and had a higher platelet count when compared with 42 JAK2V617F patients.Discussion and Conclusions: The prevalence of CALR mutation in JAK2V617F-negative ET in this study is 35.7%. HRM is an effective method of detecting CALR mutation and is a more advantageous method of screening for CALR mutation. |
| |
Keywords: | Calreticulin mutation CALR mutation type 1 CALR mutation type 2 CALR mutation JAK2V617F mutation essential thrombocythemia high-resolution melting analysis real-time polymerase chain reaction |
|
|