首页 | 本学科首页   官方微博 | 高级检索  
检索        

丹参对H2O2诱导的大鼠血管平滑肌细胞凋亡的影响
引用本文:杜先华,向虎,于龙顺,杨芳炬.丹参对H2O2诱导的大鼠血管平滑肌细胞凋亡的影响[J].中药材,2004,27(9):659-661.
作者姓名:杜先华  向虎  于龙顺  杨芳炬
作者单位:1. 郧阳医学院药理教研室,湖北,十堰,442000
2. 十堰市第四人民医院,十堰,442000
3. 四川大学基础医学与法医学院,成都,610041
摘    要:目的:探讨丹参对过氧化氢(H2O2)诱导的大鼠主动脉平滑肌细胞(VSMCs)凋亡的影响及其可能机制.方法:选用体外培养的第3~5代大鼠VSMCs,以H2O2作为外源性活性氧(ROS)诱导VSMC凋亡,用丹参进行干预,以MTT比色法测定细胞存活率,流式细胞术检测细胞凋亡率,同时用免疫细胞化学法检测VSMCs中Bcl-2和Bax蛋白表达的变化.结果:1 mmol/L H2O2诱导VSMCs凋亡,细胞存活率明显低于对照组,细胞凋亡率、Bax/Bcl-2蛋白表达的阳性指数(PI)明显高于对照组(P<0.01);丹参干预后,和H2O2组相比,细胞存活率明显升高,而细胞凋亡率、Bax/Bcl-2蛋白表达的PI显著降低(P<0.05或P<0.01).结论:丹参可通过下调Bax/Bcl-2蛋白表达而对抗H2O2诱导的VSMCs凋亡.

关 键 词:丹参  过氧化氢  平滑肌细胞  凋亡
修稿时间:2004年3月29日

Effects of Salviae miltiorrhizae on H2O2-induced Apoptosis of Rat Vascular Smooth Muscle Cells
Du Xianhua ,Xiang Hu ,Yu Longshun ,Yang Fangju.Effects of Salviae miltiorrhizae on H2O2-induced Apoptosis of Rat Vascular Smooth Muscle Cells[J].Jorunal of Chinese Medicinal Materials,2004,27(9):659-661.
Authors:Du Xianhua  Xiang Hu  Yu Longshun  Yang Fangju
Institution:Yunyang Medical College, Hubei, Shiyan 442000.
Abstract:OBJECTIVE: To investigate the effects of Salviae miltiorrhizae on H2O2-induced apoptosis in rat vascular smooth muscle cells (VSMC(s)) and its related mechanism. METHODS: The 3-5 passages of VSMC(s) were used, H2O2 was added to the medium of cultured rat aortic VSMC(s) as extraneous reactive oxygen species (ROS). The viability percentage of VSMC(s) was evaluated by MTT colorimetric assay. The percentage of apoptosis was measured by flow cytometry. The expression of Bax/Bcl-2 protein was examined with immunohistochemical technique and positive index (PI) was calculated. RESULTS: 1 mmol/L H2O2 could increase apoptosis rate. The PI of Bax/Bcl-2 protein in H2O2 group was higher (P < 0.01). When VSMC(s) were incubated with H2O2 plus Salviae miltiorrhizae, the apoptosis rate greatly declined, and at the same time, the PI of Bax/Bcl-2 protein reduced remarkably compared with H2O2 group (P < 0.05 or P < 0.01). CONCLUSION: Salviae miltiorrhizae could prevent VSMC(s) from H2O2-induced apoptosis by decreasing the Bax/Bcl-2 protein expression.
Keywords:Bax/Bcl-2
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号