川芎嗪对缺氧缺血性脑损伤大鼠海马星形胶质细胞的影响

观察川芎嗪(tetramethylpyrazine,TMP)对缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)大鼠海马星形胶质细胞的影响。将90只7日龄Wistar大鼠随机分为假手术组、HIBD组、TMP治疗组,各组分别在0h、6h、12h、24h、48h五个时间点取右脑,透射电镜下观察海马CA1区星形胶质细胞超微结构变化,免疫组织化学法检测胶质纤维酸性蛋白(glial fibrillary a-cidic protein,GFAP)表达的变化。结果显示,在电镜下HIBD损伤组星形胶质细胞肿胀,体积增大,核形状不规则,核质比增大,核仁明显,位于近核膜处,细胞器明显减少,线粒体肿胀,嵴紊乱,有缺失,可见粗面内质网池扩张;TMP组星形胶质细胞损伤轻,形态基本正常。HIBD后海马CA1区星形胶质细胞反应明显增强(P<0.01),川芎嗪治疗后可明显减轻星形胶质细胞的反应程度(P<0.01)。以上结果提示川芎嗪可通过减轻HIBD后星形胶质细胞的反应程度发挥保护和修复神经细胞的作用。

Effect of ligustrazine on hippocampal astrocytes in rats under hypoxic-ischemic brain damage

The present study aims to observe the effect of ligustrazine on hippocampal astrocytes in neonatal rats following hypoxic-ischemic brain damage. Seven-day-old wistar rats were randomly divided into three groups:sham group,HIBD group and TMP therapy group. The right half brain of each rat was isolated at the time points of 0 h、6 h、12 h、24 h、48 h. The ultrustractural changes of astrocytes in hippocampal CA1 region were observed by transmission electron microscope,and the expression of GFAP in the same region was detected with immunohistochemical method. The results showed that astrocytes in HIBD group had enlarged cell bodies,irregular nuclei,increased nuclear-cytoplasmic ratio,obvious nucleoli,located close to the nuclear membrane,decreased number of organelle,swelled mitochondria,irregular crest of mitochondria and bulged rough endoplasmic reticulum cisterna. The astrocytes in TMP therapy group were injured slightly compared with HIBD group,resembling normal astrocytic morphology. The astrocytic reactivity in hippocampal CA1 region was significantly increased in HIBD group and significantly supressed by TMP administration (P<0.01). All the results suggest that TMP can protect and repair the nerve cells due to its ability to inhibit the astrocytic reactivity induced by hypoxic-ischemic brain damage.