| K-ras基因突变在内蒙古地区结直肠癌患者中研究 |
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| 引用本文： | 韩文生,孙素青,徐伟明,娜琴,陈阿梅.K-ras基因突变在内蒙古地区结直肠癌患者中研究[J].中国优生与遗传杂志,2012(2):44-46. |
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| 作者姓名： | 韩文生 孙素青 徐伟明 娜琴 陈阿梅 |
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| 作者单位： | 内蒙古医学院组织胚胎学教研室;内蒙古医学院第四附属医院内二科 |
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| 基金项目： | 内蒙古自然科学基金资助项目(2009MS1161) |
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| 摘 要： | 目的检测内蒙地区结直肠癌K-ras基因突变情况,并结合临床病理资料加以分析。方法提取15例结直肠癌患者结直肠癌手术切除标本组织的DNA,对产物进行基因序列癌组织的DNA聚合酶链反应（PCR）扩增、DNA直接测序分析。结果 K-ras基因突变率为0%,几种分化型的结直肠癌均未发现K-ras基因突变类型,包括12密码子（GGT）、13密码子（GGC）。结论我院结直肠癌患者k-ras基因突变率为0%,转移性结直肠癌患者原发肿瘤与转移灶肿瘤k-ras基因型均相同;结直肠癌患者k-ras基因突变与否与年龄、性别、肿瘤浸润深度、肿瘤组织学类型无关。
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| 关 键 词： | 结直肠癌 K-ras基因 突变 |
K-ras mutation in colorectal cancer in Inner Mongolia |
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| HAN Wen-sheng,SUN Su-qing,XU Wei-ming,NA-qin,CHEN A-mei.K-ras mutation in colorectal cancer in Inner Mongolia[J].Chinese Journal of Birth Health & Heredity,2012(2):44-46. |
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| Authors: | HAN Wen-sheng SUN Su-qing XU Wei-ming NA-qin CHEN A-mei |
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| Affiliation: | 1.Inner Mongolia Medical College Huhohote,010050;2.The Forth Affiliated Hospital of Inner Mongolia Medical College,Baotou) |
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| Abstract: | Objective:To detect K-ras mutation in colorectal cancer in Inner Mongolia and its correlation with parameters of clinical pathology.Methods:DNA from 15 patients with colorectal carcinoma was extracted,K-ras gene was amplified by using polymerase chain reaction(PCR),and DNA direct sequencing.Mutations in exon 12 were analyzed.Results:2 kinds of K-ras gene were found,namely,codon 12(GGT),codon 13(GGC).The frequency of K-ras gene mutation in colorectal carcinoma was 0% in Inner mongolia.Conclusion:The frequency of K-ras gene mutation in colorectal carcinoma was 0% in Inner mongolia.The frequency of K-ras mutation is no correlated with the age,the position,the invasion depth,the histologic types. |
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| Keywords: | Colorectal carcinoma K-ras gene Mutation |
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