CyclinD_1基因扩增及其蛋白表达在乳腺癌中的意义

目的　探讨乳腺癌中CyclinD1基因扩增和其蛋白表达的相关性及其临床意义。方法　应用半定量PCR和免疫组化技术 ,检测和分析了乳腺癌及其癌旁组织、乳腺良性组织及正常乳腺标本中CyclinD1基因的扩增和CyclinD1蛋白的表达情况。结果　 62例乳腺癌中 ,CyclinD1基因扩增占 2 2 .6% ( 14 /62 ) ,蛋白过度表达占 48.4% ( 3 0 /62 ) ,二者有一定相关性 ,而其它各种乳腺组织的CyclinD1基因扩增及蛋白过度表达与之相比差异有显著性意义 (P <0 .0 5 )。Cy clinD1基因扩增及蛋白过度表达与乳腺癌组织学分级呈明显正相关 ,且CyclinD1蛋白过度表达还与雌孕激素受体状况呈正相关。结论　乳腺癌中CyclinD1基因扩增程度和其蛋白过度表达虽有一定相关性 ,但并不完全一致 ,说明还存在着其它导致CyclinD1蛋白过度表达的机理 ,CyclinD1蛋白的表达可能还受雌激素的调控。对乳腺癌中CyclinD1基因及其蛋白表达的检测有一定临床意义。

AMPLIFICATION OF CYCLIND_1 GENE AND OVEREXPRESSION OF ITS PROTEIN PRODUCT IN BREAST CANCER

Objective To investigate the clinical significance of the amplification of CyclinD 1 gene and overexpression of its protein product in breast cancer. Methods Semi quantitative PCR and immunohistochemical techniques were used to examine the amplification of CyclinD 1 gene and overexpression of its protein product in breast cancer, mammary tissues adjacent to the tumor, mammary benign disease and normal mammary tissues. Results The results showed that amplification of CyclinD 1 gene was observed in 14 out of 62 (22.6%) breast cancer, the overexpression of its protein product was 48.4% (30/62). Compared with other mammary tissues, these findings were significant. Amplification of CyclinD 1 gene and overexpression of its protein were positively associated with histological grade, and there was a strong correlation between CyclinD 1 protein overexpression and estrogen receptor (progestogen receptor). Conclusion The degree of CyclinD 1 protein overexpression is not quite the same as the frequency of the amplification of CyclinD 1 gene. It suggests that the overexpresstion of CyclinD 1 protein is also attributed to some other mechanisms. Estrogen might stimulate the expression of CyclinD 1. The detection of CyclinD 1 gene and its protein in breast cancer has some clinical significances.