异位性皮炎患者循环T细胞激活表型和化学趋化功能研究

目的：为了明确异性皮炎患者循环Ｔ细胞的体外细胞生物学功能，和阐明Ｔ细胞在异位性皮炎发病过程中的可能作用。方法：运用４８孔微型化学趋化装置，测定２１例异位性皮炎患者与２０例正常人的纯化Ｔ细胞对白细胞介素８（ＩＬ－８）的化学趋化反应，同时用单克隆抗体荧光免疫法在流式细胞仪上测定Ｔ细胞表面ＨＬＡ－ＤＡ和白细胞介素２受体（ＩＬ－２Ｒ）等激活表型，以及用ＥＳＫＳＡ法测定血肖ＩＬ－８水平。结果：（１）异们性皮

T-lymphocyte Activation and Chemotaxis in Patients with Atopic Dermatitis

Objective:In order to determine whether circulating T cells from patients with atopic dermatitis were also involved in the disease process.Methods:The authors investigated T cell activation markers and the biological behavior.A 48 microchemtaxis chamber was employed to determine T cell chemotaxis activity in 21 patients with atopic dermatitis and 20 normal controls;the expression of T cell activation markers HLA DR and interleukin 2 receptor (IL 2R) were analysed with fluorescence activated cell sorting technique and serum interleukin 8 (IL 8) levels were measured with ELISA method.Results:T cell chemotactic responses were significantly decreased in patients with atopic dermatitis compared with healthy controls.Increased expression of HLA DR and IL 2R were demonstrated in circulating T cells from those patients comparied with healthy controls.Serum IL 8 levels were found significantly increased in patients with atopic dermatitis.Conclusion:Circulating T cells in patients with atopic dermatitis showed abnormal chemotactic response in vitro,which may be associated with activation state in vivo and increased serum IL 8 levels of these patients.The abnormal T cell chemotactic response may be involved in the pathogenesis of atopic dermatitis.